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Antibodies > ABCG5 > LS-C313009
 
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Catalog Number Size Price
LS-C313009-10 10 µg $318 
LS-C313009-100 100 µg (0.5 mg/ml) $470 
ABCG5 Antibody - Western blot analysis of ABCG5 using anti-ABCG5 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate Lane 2: A549 Whole Cell Lysate Lane 3: HT1080 Whole Cell Lysate Lane 4: U87 Whole Cell Lysate Lane 5: SKOV Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCG5 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABCG5 at approximately 73KD. The expected band size for ABCG5 is at 73KD.
ABCG5 Antibody - WB of ABCG5 antibody. Lane 1: MCF-7 Cell Lysate. Lane 2: A549 Cell Lysate. Lane 3: HT1080 Cell Lysate. Lane 4: U87 Cell Lysate. Lane 5: SKOV Cell Lysate..
ABCG5 Antibody - Flow Cytometry analysis of A431 cells using anti-ABCG5 antibody. Overlay histogram showing A431 cells stained with anti-ABCG5 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCG5 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ABCG5 Antibody - Western blot analysis of ABCG5 using anti-ABCG5 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate Lane 2: A549 Whole Cell Lysate Lane 3: HT1080 Whole Cell Lysate Lane 4: U87 Whole Cell Lysate Lane 5: SKOV Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCG5 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABCG5 at approximately 73KD. The expected band size for ABCG5 is at 73KD.
ABCG5 Antibody - WB of ABCG5 antibody. Lane 1: MCF-7 Cell Lysate. Lane 2: A549 Cell Lysate. Lane 3: HT1080 Cell Lysate. Lane 4: U87 Cell Lysate. Lane 5: SKOV Cell Lysate..
ABCG5 Antibody - Flow Cytometry analysis of A431 cells using anti-ABCG5 antibody. Overlay histogram showing A431 cells stained with anti-ABCG5 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCG5 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human ABCG5 Antibody (aa116‑131, WB) LS‑C313009

Polyclonal Rabbit anti‑Human ABCG5 Antibody (aa116‑131, WB) LS‑C313009

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Note: This antibody replaces LS-C387926
Antibody:
ABCG5 Rabbit anti-Human Polyclonal (aa116-131) Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313009-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ABCG5 Rabbit anti-Human Polyclonal (aa116-131) Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ABCG5 antibody LS-C313009 is an unconjugated rabbit polyclonal antibody to human ABCG5 (aa116-131). Validated for WB.
Target
Human ABCG5
Synonyms
ABCG5 | STSL | Sterolin | Sterolin 1 | Sterolin-1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human ABCG5(116-131 aa NGRALRREQFQDCFSY).
Epitope
aa116-131
Specificity
Strongly expressed in the liver, lower levels in the small intestine and colon.
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ABCG5
Q9H222 NM_022436 NP_071881.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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