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Anti-ABCG1 Antibody (aa300-400, Biotin) LS-C83841

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ABCG1 Antibodies

Anti-ABCG1 Antibody (aa79-312) LS-C292948
Rabbit Polyclonal (IgG) to Human ABCG1
Human
Western blot, ELISA
Unconjugated
Western blot Image
Anti-ABCG1 Antibody (aa627-647) LS-C313008
Rabbit Polyclonal (IgG) to Human ABCG1
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Rabbit, Sheep
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ABCG1
Human, Mouse
IHC, IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Biotin Conjugated

Details

Human ABCG1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Biotin. Also available Unconjugated or conjugated with DY650, DY488, DY550.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)

  • Immunoprecipitation

Specificity and Use

ABCG1 antibody was raised against a synthetic peptide made to an internal region of human ABCG1 (between residues 300-400)
aa300-400
The specificity of this is for ABCG1.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. This antibody is useful for Western blot on mouse peritoneal macrophages treated with 22-R-hydroxycholesterol and T0901317 (stimulating ABCG1 expression) and immunohistochemistry. The theoretical molecular weight of ABCG1 is ~75.6 kD, as seen by Western blot. Preliminary results suggest that this antibody may be also used for immunofluorescence on mouse ABCG1 overexpressed in HEK93 cells. It is not recommended for immunoprecipitation.

Packaging

Antiserum
Store at 4°C. Do not freeze.
For research use only.

About ABCG1

P45844 NM_004915 NP_004906.3

ABCG1 Antibody, ABC transporter 8 Antibody, ABC transporter Antibody, ABC8 Antibody, Homolog of Drosophila white Antibody, White protein homolog Antibody, WHITE1 Antibody, WHT1 Antibody

ABCG1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types.

Immunohistochemistry

Immunohistochemistry
Anti-ABCG1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B507 concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Detection of ABCG1 in mouse peritoneal macrophages (40 ug). Lane 1: positive ABCG1 control, lane 2: 10 uM 22-(R)-hydrocholesterol treated macrophages, Lane 3: 100 nM T0901713 (LXRa ligand) treated macrophages, and Lane 4: 500 nM T091713 (LXRa ligand) treated macrophages. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Detection of ABCG1 in mouse peritoneal macrophages (40 ug), using LS-C2757. Lane 1: positive ABCG1 control, lane 2: 10 uM 22-(R)-hydrocholesterol treated macrophages, Lane 3: 100 nM T0901713 (LXRa ligand) treated macrophages, and Lane 4: 500 nM T091713 (LXRa ligand) treated macrophages. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/5/2016

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