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Anti-ABCB5 Antibody (Internal) LS-C154236

Ordering

Wt. Vol. Conc. Price
100 µg - 1.29 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ABCB5 Antibodies

Anti-ABCB5 Antibody IHC-plus™ LS-B6371
Rabbit Polyclonal (IgG) to Human ABCB5
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ABCB5 Antibody (aa1-30) IHC-plus™ LS-B3454
Rabbit Polyclonal to Human ABCB5
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-ABCB5 Antibody (clone 5H3C6) LS-C169144
Mouse Monoclonal [clone 5H3C6] (IgG1) to Human ABCB5
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human ABCB5
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human ABCB5
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:200)
  • Western blot (1:20000)
  • ELISA

Specificity and Use

ABCB5 antibody was raised against aBCB5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5.
Internal
This antibody is specific for human ABCB5 protein. A BLAST analysis was used to suggest partial cross-reactivity with ABCB5 from monkey (85% homology), rat (68% homology) and mouse (62% homology) sources. Cross-reactivity with ABCB5 from other sources has not been determined.
This affinity purified ABCB5 antibody has been tested for use in ELISA, IHC, and western blotting. Specific conditions for reactivity should be optimized by the end user.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About ABCB5

Q2M3G0 NM_178559 NP_848654.3

ABCB5 Antibody, ABCB5alpha Antibody, ABCB5 P-gp Antibody, ABCB5beta Antibody, ATP-binding cassette protein Antibody, EST422562 Antibody, P-glycoprotein ABCB5 Antibody

Drug efflux transporter present in a number of stem cells that acts as a regulator of cellular differentiation. Able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.

Immunohistochemistry

Immunohistochemistry
IHC - ABCB5 Antibody. Immunohistochemistry of ABCB5 antibody tissue: human liver Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized Primary antibody: ABCB5 1:200 Secondary antibody: Peroxidase goat anti-rabbit at 1:10000 for 45 min at RT Localization: Moderate to strong cytoplasmic and membranous staining was observed in hepatocytes. Occasional nuclear staining was observed in hepatocytes and sinusoidal cells. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Western blot

Western blot
Western Blot - ABCB5 Antibody. Western blot of affinity purified anti-ABCB5 antibody shows detection of ABCB5 beta in ~12.5 ug of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kD ABCB5. The membrane was probed with the primary antibody at a 1:10000 dilution in 5% milk in TBST at 4C, overnight. Personal Communication, JP Gillet, CCR-NCI, Bethesda, MD.

Requested From: United States
Date Requested: 12/8/2016

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