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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ABCB5 Antibody (DY550) LS‑C180807
ABCB5 antibody LS-C180807 is a DY550-conjugated rabbit polyclonal antibody to ABCB5 from human and mouse. Validated for ICC and IHC.
Catalog
Size
Price
LS-C180807-100
100 µl (1 mg/ml)
Unavailable

Popular ABCB5 Products

Anti-ABCB5 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3454 concentration 10 ug/ml.
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Anti-ABCB5 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6371 concentration 2.5 ug/ml.
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IHC - ABCB5 Antibody. Immunohistochemistry of ABCB5 antibody tissue: human liver Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized Primary antibody: ABCB5 1:200 Secondary antibody: Peroxidase goat anti-rabbit at 1:10000 for 45 min at RT Localization: Moderate to strong cytoplasmic and membranous staining was observed in hepatocytes. Occasional nuclear staining was observed in hepatocytes and sinusoidal cells. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
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Immunohistochemistry: ABCB5 Antibody - Analysis of ABCB5 in mouse spleen using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC

Product Description

ABCB5 antibody LS-C180807 is a DY550-conjugated rabbit polyclonal antibody to ABCB5 from human and mouse. Validated for ICC and IHC.
About ABCB5
Drug efflux transporter present in a number of stem cells that acts as a regulator of cellular differentiation. Able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair. Q2M3G0 NM_178559 NP_848654.3

ABCB5 Antibody, ABCB5alpha Antibody, ABCB5 P-gp Antibody, ABCB5beta Antibody, ATP-binding cassette protein Antibody, EST422562 Antibody, P-glycoprotein ABCB5 Antibody


Specifications

Target
Human ABCB5
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
DyLight 550. Also available Unconjugated or conjugated with Biotin, DY650, DY488.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • ICC (1:100)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ABCB5 antibody was raised against a synthetic peptide made to an internal portion of the human ABCB5 protein (between residues 150-200) [UniProt Q2M3G0].
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM sodium borate
Storage
Store at 4°C in the dark.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Immunohistochemistry: ABCB5 Antibody - Analysis of ABCB5 in mouse spleen using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: ABCB5 Antibody - Analysis of ABCB5 in mouse spleen using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: ABCB5 Antibody - ABCB5 antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: ABCB5 Antibody - ABCB5 antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry: ABCB5 Antibody - Analysis of ABCB5 in mouse spleen using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: ABCB5 Antibody - Analysis of ABCB5 in mouse spleen using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: ABCB5 Antibody - ABCB5 antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: ABCB5 Antibody - ABCB5 antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry: ABCB5 Antibody - Analysis of ABCB5 in mouse spleen using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: ABCB5 Antibody - Analysis of ABCB5 in mouse spleen using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: ABCB5 Antibody - ABCB5 antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: ABCB5 Antibody - ABCB5 antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry: ABCB5 Antibody - Analysis of ABCB5 in mouse spleen using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: ABCB5 Antibody - Analysis of ABCB5 in mouse spleen using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: ABCB5 Antibody - ABCB5 antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: ABCB5 Antibody - ABCB5 antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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