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Anti-ABCB5 Antibody (clone 5H3C6) LS-C169144

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ABCB5 Antibodies

Anti-ABCB5 Antibody IHC-plus™ LS-B6371
Rabbit Polyclonal (IgG) to Human ABCB5
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ABCB5 Antibody (aa1-30) IHC-plus™ LS-B3454
Rabbit Polyclonal to Human ABCB5
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-ABCB5 Antibody (clone 5H3C6) LS-C169144
Mouse Monoclonal [clone 5H3C6] (IgG1) to Human ABCB5
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 5H3C6] (IgG1) to Human ABCB5
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human ABCB5
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [5H3C6]
Unconjugated
Purified
Unmodified

Applications

  • IHC - Paraffin (1:200 - 1:1000)
  • ICC (1:200 - 1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)

Specificity and Use

ABCB5 antibody was raised against purified recombinant fragment of human ABCB5 (aa481-674) expressed in E. coli.
Human ABCB5

Packaging

PBS, 0.05% sodium azide, 0.05% protein stabilizer
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About ABCB5

Q2M3G0 NM_178559 NP_848654.3

ABCB5 Antibody, ABCB5alpha Antibody, ABCB5 P-gp Antibody, ABCB5beta Antibody, ATP-binding cassette protein Antibody, EST422562 Antibody, P-glycoprotein ABCB5 Antibody

Drug efflux transporter present in a number of stem cells that acts as a regulator of cellular differentiation. Able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded liver cancer tissues using ABCB5 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded breast cancer tissues using ABCB5 mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence
Immunofluorescence of HepG2 cells using ABCB5 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot
Western blot using ABCB5 monoclonal antibody against human ABCB5 recombinant protein. (Expected MW is 47 kDa)

Western blot

Western blot
Western blot using ABCB5 mouse monoclonal antibody against A431 (1) cell lysate.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Requested From: United States
Date Requested: 12/11/2016

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