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Anti-AAK1 Antibody (aa677-691) LS-C112877

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular AAK1 Antibodies

Anti-AAK1 Antibody (N-Terminus) IHC-plus™ LS-B3843
Rabbit Polyclonal to Human AAK1
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-AAK1 Antibody (aa704-822, clone 12K1) LS-C182750
Mouse Monoclonal [clone 12K1] (IgG2b) to Human AAK1
Human
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human AAK1
Human, Monkey, Bat, Horse
Western blot, ELISA
Unconjugated

Details

Human AAK1
Goat
Human, Monkey, Bat, Horse (tested or 100% immunogen sequence identity)
Bovine, Dog, Pig, Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1 - 3 µg/ml)
  • ELISA (1:16000)

Specificity and Use

AAK1 antibody was raised against synthetic peptide C-GSPRTSQQNVYNPSE from an internal region of human AAK1 (NP_055726.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Bat, Horse, Opossum (100%); Dog, Bovine, Rabbit, Pig (93%); Mouse, Rat, Hamster, Panda, Turkey, Chicken, Platypus, Xenopus (87%).
LS-E27179 - Lyophilized - 100 µg - $145.00
aa677-691
Human AAK1.
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 100kDa band observed in Human Brain (Amygdala and Cerebellum) lysates (calculated MW of 104kDa according to NP_055726.3). Recommended concentration: 1-3 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About AAK1

Q2M2I8 NM_014911 NP_055726.3

AAK1 Antibody, Adaptor-associated kinase 1 Antibody, AP2 associated kinase 1 Antibody, KIAA1048 Antibody

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes.

Western blot

Western blot
AAK1 antibody (1 ug/ml) staining of Human Amygdala lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/2/2016

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