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Anti-5-mC / 5-Methylcytosine Antibody (Azide-free) LS-C187857


Wt. Vol. Conc. Price
100 µg - 1 mg/ml Unavailable

Most Popular 5-mC / 5-Methylcytosine Antibodies

Anti-5-mC / 5-Methylcytosine Antibody LS-C84008
Sheep Polyclonal
ICC, Immunofluorescence, Western blot, Immunoprecipitation
Anti-5-mC / 5-Methylcytosine Antibody (clone 13A149) LS-C183366
Mouse Monoclonal [clone 13A149] (IgG1,l)
Dot Blot
Anti-5-mC / 5-Methylcytosine Antibody (Azide-free) LS-C187857
Sheep Polyclonal (IgG) to Broad 5-mC / 5-Methylcytosine
Broad, Rat
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated, Azide-free
Anti-5-mC / 5-Methylcytosine Antibody (AP) LS-C502468
Mouse Monoclonal (IgG1,l) to Human 5-mC / 5-Methylcytosine
Dot Blot
AP Conjugated
Anti-5-mC / 5-Methylcytosine Antibody (APC) LS-C512174
Mouse Monoclonal (IgG1,l) to Human 5-mC / 5-Methylcytosine
Dot Blot
APC Conjugated

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Broad 5-mC / 5-Methylcytosine
Broad, Rat
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated, Azide-free


Broad 5-mC / 5-Methylcytosine
Broad, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Affinity purified


  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen (10 µg/ml)
  • Immunofluorescence
  • Western blot (1:1000)
  • Immunoprecipitation (25 - 100 µg/ml)

Specificity and Use

Keyhole limpet hemocyanin (KLH) conjugated 5-methylcytosine.
Sheep Anti 5-Methylcytosine (5-MeC) recognizes the methylated form of cytosine (C), an epigenetic modification catalyzed by DNA methyltransferases resulting in the attachment of a methyl group to carbon 5. This modification alters the structure of cytosine but not its base-pairing properties. The timing and function of cytosine methylation varies amongst species with roles in such processing events as transcription and recombination. For example, in mammals, methylation occurring during early development and emrbyogensis functions in X chromosome inactivation and gene imprinting, whereas in plants, DNA methylation occurs later and affects genomic stability through silencing of transposable elements. The deamination of 5-MeC results in the formation of thymine (T) instead of uracil. Since thymine is not recognized by DNA repair enzymes, cytosine to thymine conversions can cause transition mutations. The most frequent mutations in human genetic diseases are those involving C to T transitions occurring at CG sites. Another type of mutation involving 5-MeC involves the formation of pyrimidine dimers. Upon exposure to sunlight, pyrimidine dimers form preferentially at dipyrimidine sequences containing 5-MeC, a mutation frequently seen in human skin cancers. Sheep Anti-5-MeC is reported as suitable for use in immunocytochemistry.
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.


Store at -20°C. Minimize freezing and thawing.
For research use only.

About 5-mC / 5-Methylcytosine

Requested From: 
Date Requested: 4/25/2017

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