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Anti-5-mC / 5-Methylcytosine Antibody (Azide-free) LS-C187857

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $310
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular 5-mC / 5-Methylcytosine Antibodies

Anti-5-mC / 5-Methylcytosine Antibody LS-C84008
Sheep Polyclonal
ICC, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Anti-5-mC / 5-Methylcytosine Antibody (clone 13A149) LS-C183366
Mouse Monoclonal [clone 13A149] (IgG1,l)
Human
Dot Blot
Unconjugated

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Broad 5-mC / 5-Methylcytosine
Broad, Rat
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated, Azide-free

Details

Broad 5-mC / 5-Methylcytosine
Sheep
Broad, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Affinity purified
Azide-free

Applications

  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen (10 µg/ml)
  • Immunofluorescence
  • Western blot (1:1000)
  • Immunoprecipitation (25 - 100 µg/ml)

Specificity and Use

Keyhole limpet hemocyanin (KLH) conjugated 5-methylcytosine.
Sheep Anti 5-Methylcytosine (5-MeC) recognizes the methylated form of cytosine (C), an epigenetic modification catalyzed by DNA methyltransferases resulting in the attachment of a methyl group to carbon 5. This modification alters the structure of cytosine but not its base-pairing properties. The timing and function of cytosine methylation varies amongst species with roles in such processing events as transcription and recombination. For example, in mammals, methylation occurring during early development and emrbyogensis functions in X chromosome inactivation and gene imprinting, whereas in plants, DNA methylation occurs later and affects genomic stability through silencing of transposable elements. The deamination of 5-MeC results in the formation of thymine (T) instead of uracil. Since thymine is not recognized by DNA repair enzymes, cytosine to thymine conversions can cause transition mutations. The most frequent mutations in human genetic diseases are those involving C to T transitions occurring at CG sites. Another type of mutation involving 5-MeC involves the formation of pyrimidine dimers. Upon exposure to sunlight, pyrimidine dimers form preferentially at dipyrimidine sequences containing 5-MeC, a mutation frequently seen in human skin cancers. Sheep Anti-5-MeC is reported as suitable for use in immunocytochemistry.
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Packaging

PBS
Store at -20°C. Minimize freezing and thawing.
For research use only.

About 5-mC / 5-Methylcytosine


Requested From: United States
Date Requested: 12/6/2016

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