Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Recognizes rat 5-Hydroxytryptophan. See additional cross-reactivity data.
Suitable for use in Immunohistochemistry, ELISA. Immunohistochemistry: 1:2000-1:5000. Tested using the free floating PAP technique on rat raphe nuclei. The anti-conjugated 5-hydroxytryptophan antibodies stains well. Additional cross-reactivity data: Using a conjugate 5 hydroxytryptophan-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compounds Cross-reactivity ratio (a). 5-Hydroxytryptophan-G-BSA1. 5-Methoxytryptophan-G-BSA1:2000. Tryptophan-G-BSA1:50000. 5-Hydroxytryptamine-G-BSA1:50000. Tryptamine-G-BSA1:>50000. 5-Hydroxytryptophan1:>50000. (a): 5-Hydroxytryptophan-G-BSA concentration/unconjugated or conjugated indolealkylamine concentration at half displacement. G = Glutaraldehyde, BSA. PAP procedure: Second antibody: Sections are incubated with 1/100 dilution of goat anti-rabbit in solution C for 3 hours at 20?C or 1 hour at 37?C. Then, they are washed 3 times (10 min) with solution C. PAP: Sections are incubated with 1/1000 dilution of rabbit peroxidase anti-peroxidase complex in solution C for 1 hour at 37?C. Then, they are washed 3 times (10 min) with solution C. Antibody-antigen complexes are revealed using diaminobenzidine (25 mg/100ml) (or other chromagen) dissolved in Tris 0.05 M and filtrated ; 0.05% of H2O2 is added. The sections are incubated for 10 min at 20?C. Reaction is stopped by transferring sections in 5 ml of Tris 0.05 M.