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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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5‑Alpha Reductase / SRD5A1 Antibody (aa2‑13) IHC‑plus™ LS‑B1764 Best Seller
Note: This antibody replaces LS-C47560
SRD5A1 antibody LS-B1764 is an unconjugated goat polyclonal antibody to human SRD5A1 (5-Alpha Reductase). Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1764-50
50 µg (0.5 mg/ml)
Unavailable

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Product Description

SRD5A1 antibody LS-B1764 is an unconjugated goat polyclonal antibody to human SRD5A1 (5-Alpha Reductase). Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
About 5-Alpha Reductase / SRD5A1
Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. P18405 NM_001047 NP_001038.1

SRD5A1 Antibody, 5-alpha reductase Antibody, SR type 1 Antibody, Steroid 5-alpha-reductase 1 Antibody, S5AR 1 Antibody


Specifications

Target
Human 5-Alpha Reductase / SRD5A1
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide ATATGVAEERLLC from the N-terminus of human SRD5A1 (NP_001038.1). Percent identity by BLAST analysis: Human, Gibbon (100%); Gorilla, Marmoset (92%); Ajellomyces (83%).
Blocking Peptide
LS-E25823 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1764. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa2-13
Specificity
Human SRD5A1.
Usage
Immunohistochemistry: LS-B1764 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1764 was determined to be 2.5 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-SRD5A1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1764 concentration 5 ug/ml.
Anti-SRD5A1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1764 concentration 5 ug/ml.

Immunohistochemistry

Anti-SRD5A1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1764 concentration 5 ug/ml.
Anti-SRD5A1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1764 concentration 5 ug/ml.

Immunohistochemistry

Anti-SRD5A1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1764 concentration 5 ug/ml.
Anti-SRD5A1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1764 concentration 5 ug/ml.

Immunohistochemistry

Anti-SRD5A1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1764 concentration 5 ug/ml.
Anti-SRD5A1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1764 concentration 5 ug/ml.


Requested From: 
Date Requested: 6/19/2018

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