Human 4E-BP2 / EIF4EBP2

Rabbit

Human, Mouse, Rat, Bovine, Dog, Pig (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Human PHAS-II synthetic peptide (aa 99-120) was synthesized and the peptide coupled to KLH. This sequence is 96% homologous to mouse PHAS-11 over these residues

aa99-120

Detects 17 and 20 kD proteins, corresponding to the apparent molecular mass of PHAS-II and its phosphorylated state on SDS-PAGE immunoblots.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot (4 µg/ml)

Immunohistochemistry: LS-B3749 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3749 was determined to be 10 ug/ml.

25 mM Sodium Borate, BBS, pH 8.4, 75 mM NaCl, 5 mM EDTA, 100 mM Boric acid

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q13542 NCBI: BC005057 NP_004087.1