14-3-3 Binding Motif

Mouse

IgG1 Monoclonal

Unconjugated

Ascites

Unmodified

Synthetic phospho-(Ser) 14-3-3 binding motif peptides (KLH coupled).

pSer

Binds peptides/ proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. By ELISA, recognizes a wide range of peptides containing this phosphorylated 14-3-3 binding motif in a manner that is phospho-specific and largely independent of the surrounding amino acid sequence. Weakly cross-reacts with sequences containing phospho-Thr instead of phospho-Ser in this motif and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No cross-reactivity is observed with corresponding non-phosphorylated sequences.

• Western blot (1:4000)
• Immunoprecipitation (1:20)
• ELISA (1:1000)

Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:4000. Immunoprecipitation: 1:20 (Denatured proteins). ELISA: 1:1000.

10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol

For research use only. Intended for use by laboratory professionals.

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