Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position. Antibody binding is phospho-specific and largely independent of other surrounding amino acids. Weakly cross-reacts with sequences containing phospho-Thr instead of phospho-Ser in this motif; with sequences containing phospho-Ser surrounded by Phe at the +1 position; with Arg/Lys at the -3 position. No cross-reactivity is observed with corresponding non-phosphorylated sequences or with other phospho- Thr/Ser/Tyr -containing motifs.
Suitable for use in, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:1000. By 2D gel Western Blot, recognizes a large number of presumptive 14-3-3 binding proteins. Immunoprecipitation: 1:50. Immunohistochemistry (Paraffin): 1:200. ELISA: 1:1000. Recognizes a wide range of peptides containing the 14-3-3 binding motif.
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol.