14-3-3 Binding Motif

Rabbit

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic phospho- (Ser) 14-3-3-binding-motif peptides.

pSer

Binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position. Antibody binding is phospho-specific and largely independent of other surrounding amino acids. Weakly cross-reacts with sequences containing phospho-Thr instead of phospho-Ser in this motif; with sequences containing phospho-Ser surrounded by Phe at the +1 position; with Arg/Lys at the -3 position. No cross-reactivity is observed with corresponding non-phosphorylated sequences or with other phospho- Thr/Ser/Tyr -containing motifs.

• IHC
• IHC - Paraffin
• Western blot (1:1000)
• Immunoprecipitation (1:50)
• ELISA (1:1000)

Suitable for use in, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:1000. By 2D gel Western Blot, recognizes a large number of presumptive 14-3-3 binding proteins. Immunoprecipitation: 1:50. Immunohistochemistry (Paraffin): 1:200. ELISA: 1:1000. Recognizes a wide range of peptides containing the 14-3-3 binding motif.

10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.