14-3-3 antibody LS-C63153 is an unconjugated mouse monoclonal antibody to 14-3-3 from human, mouse, rat and other species. Validated for ICC, IHC and WB.
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Sheep
(tested or 100% immunogen sequence identity)
Protein G purified
- IHC (1:50)
- Western blot
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
14-3-3 antibody was raised against lysed transformed human amnion cells (AMA).
Detects ~29-30 kD proteins, corresponding to the apparent molecular mass of the beta and zeta isoforms of 14-3-3 on SDS-PAGE immunoblots, in samples from human, mouse, rat, monkey, rabbit, dog, sheep, pig and bovine origins. This antibody is suitable as a Golgi marker as the epitopes recognized are on the cytoplasmic face of the Golgi membrane. It also reacts specifically with two members of the 14-3-3 protein family: the acidic human IEF58 (~29 kD) and IEF57a (~30 kD), which correspond to 14-3-3 beta and zeta, respectively.
PBS, pH 7.2, 0.1 mM PMSF, 0.09% Sodium Azide, 50% Glycerol
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.