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Catalog Number Size Price
LS-C662031-10 10 µg $318 
LS-C662031-100 100 µg $470 
ANGPT1 / Angiopoietin-1 Antibody - Western blot analysis of Angiopoietin-1 using anti-Angiopoietin-1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human ANGPT1 protein 1ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin-1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Angiopoietin-1 at approximately 70KD. The expected band size for Angiopoietin-1 is at 57KD.

Monoclonal Mouse anti‑Human ANGPT1 / Angiopoietin‑1 Antibody (WB) LS‑C662031

Monoclonal Mouse anti‑Human ANGPT1 / Angiopoietin‑1 Antibody (WB) LS‑C662031

Antibody:
ANGPT1 / Angiopoietin-1 Mouse anti-Human Monoclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662031-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ANGPT1 / Angiopoietin-1 Mouse anti-Human Monoclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Angiopoietin-1 antibody LS-C662031 is an unconjugated mouse monoclonal antibody to human Angiopoietin-1 (ANGPT1). Validated for ELISA and WB.
Target
Human ANGPT1 / Angiopoietin-1
Synonyms
ANGPT1 | Angiopoietin 1 | Angiopoietin-1 | AGPT | AGP1 | ANG-1 | ANG1 | KIAA0003
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Angiopoietin-1 (204-241 aa KHKEELDTLKEEKENLQGLVTRQTYIIQELEKQLNRAT), different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ANGPT1 / Angiopoietin-1
Q15389 NM_001146 NP_001137.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024