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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C19022-100 100 µl (85 mg/ml) $425 
Antibody

Polyclonal ANAPC11 / APC11 Antibody (aa76‑84, IHC, WB) LS‑C19022

Polyclonal ANAPC11 / APC11 Antibody (aa76‑84, IHC, WB) LS‑C19022

Antibody:
Rabbit Polyclonal to Human ANAPC11 / APC11
Application:
IHC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C19022-100
100 µl
85 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human ANAPC11 / APC11
Application:
IHC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
APC11 antibody LS-C19022 is an unconjugated rabbit polyclonal antibody to human APC11 (ANAPC11). Validated for ELISA, IHC, IP and WB. Cited in 2 publications.
Target
Human ANAPC11 / APC11
Synonyms
ANAPC11 | Apc11p | APC11 | Cyclosome subunit 11 | HSPC214
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated followed by sterile filtration
Modifications
Unmodified
Applications
  • IHC
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 76-84 of Human APC11 (C-terminal) coupled to KLH.
Epitope
aa76-84
Specificity
Reacts with human and mouse APC11. Cross reactivity may also occur with APC11 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and ROC2.
Usage
This antibody reacts with human APC11 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins (APC2) does occur. A 9.8 kD band corresponding to human APC11 is detected. Most cell lines or tissues expressing APC11 can be used as a positive control. Researchers should determine optimal titers for other applications.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ANAPC11 / APC11
Q9NYG5 NM_016476 NP_057560.8

LSBio Ratings

ANAPC11 / APC11 Antibody (aa76-84) for IHC, WB/Western, IP, ELISA LS-C19022 has an LSBio Rating of
Publications (4.1)

Publications (2)

Association with cullin partners protects ROC proteins from proteasome-dependent degradation. Ohta T, Michel JJ, Xiong Y. Oncogene. 1999 18:6758-66.

Activation of UBC5 ubiquitin-conjugating enzyme by the RING finger of ROC1 and assembly of active ubiquitin ligases by all cullins. Furukawa M, Ohta T, Xiong Y. The Journal of biological chemistry. 2002 277:15758-65.

Customer Reviews (0)


Requested From: United States
Date Requested: 2/28/2020