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ANAPC1 / APC1 Antibody (phospho‑Ser355) LS‑C18983

Anti-APC1 pS355 Antibody - Western Blot. Western blot of Affinity Purified anti-APC1 pS355 antibody shows detection of a band ~215 kD corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with thymidine to synchronize cells at the G1/S boundary. Lane 3 shows lysate from cells treated with nocodazole to synchronize cells at the M phase. Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division. Each lane contains approximately 30 ug of HeLa S3 whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose. After blocking with 5% non-fat dry milk in TTBS, the membrane was probed with the primary antibody diluted to 1:500 for 1 h at room temperature followed by washes and reaction with a 1:5000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX (LS-C60865) for 45 min at room temperature. ECL reagent was used for detection. Other detection systems will yield similar results. Data contributed by Bing Li, UT Southwestern.

ANAPC1 / APC1 Antibody (phospho‑Ser355) LS‑C18983

Antibody:
Rabbit Polyclonal to Human ANAPC1 / APC1
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C18983-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human ANAPC1 / APC1
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
APC1 antibody LS-C18983 is an unconjugated rabbit polyclonal antibody to APC1 (ANAPC1) from human, mouse, rat and other species. Validated for ELISA and WB.
Target
Human ANAPC1 / APC1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:200 - 1:2000)
  • ELISA (1:10000 - 1:40000)
Immunogen
ANAPC1 / APC1 antibody was raised against synthetic peptide from human ANAPC1.
Epitope
pSer355
Specificity
Amino acids 351-359 of Human Apc1 protein.
Usage
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band - 215 kD in size corresponding to APC1 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ANAPC1 / APC1
Q9H1A4 NM_022662 NP_073153.1

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Product
Species: Human, Mouse, Rat
Applications: Western blot, Immunoprecipitation
Product
Species: Human
Applications: Western blot, ELISA
Reactivity: Rat
Range: 15.63-1000 pg/ml
Reactivity: Chicken
Range: 0.16-10 ng/ml

Requested From: United States
Date Requested: 7/22/2019
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