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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AMPH / Amphiphysin Antibody (clone 3) IHC‑plus™ LS‑B3757 Best Seller
Note: This antibody replaces LS-C63306
Amphiphysin antibody LS-B3757 is an unconjugated mouse monoclonal antibody to Amphiphysin (AMPH) from human, mouse, rat and other species. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3757-50
50 µg (1 mg/ml)
$425
Description
Amphiphysin antibody LS-B3757 is an unconjugated mouse monoclonal antibody to Amphiphysin (AMPH) from human, mouse, rat and other species. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human AMPH / Amphiphysin
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal [3]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.5 µg/ml)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human Amphiphysin.
Specificity
Detects a ~128 kD protein, corresponding to the apparent molecular mass of Amphiphysin I on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, hamster, monkey and rabbit origins. A 108 kD isoform may be observed in some tissues or cell lysates. The epitope is located within the carboxyl terminal region of Amphiphysin I (residues 545-695). Rat brain immunostaining using this antibody in immunofluorescence shows synaptic distribution.
Usage
Immunohistochemistry: LS-B3757 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3757 was determined to be 10 ug/ml.
Presentation
PBS, 0.1 mM PMSF, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
About AMPH / Amphiphysin
P49418 NM_001635 NP_001626.1

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Immunohistochemistry

Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunohistochemistry

Anti-AMPH / Amphiphysin antibody IHC of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-AMPH / Amphiphysin antibody IHC of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunohistochemistry

Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunohistochemistry

Anti-AMPH / Amphiphysin antibody IHC of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-AMPH / Amphiphysin antibody IHC of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunohistochemistry

Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunohistochemistry

Anti-AMPH / Amphiphysin antibody IHC of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-AMPH / Amphiphysin antibody IHC of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunohistochemistry

Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunohistochemistry

Anti-AMPH / Amphiphysin antibody IHC of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-AMPH / Amphiphysin antibody IHC of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Requested From: United States
Date Requested: 1/18/2019
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