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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AMPH / Amphiphysin Antibody (aa1‑194) LS‑C185530
Amphiphysin antibody LS-C185530 is an unconjugated rabbit polyclonal antibody to Amphiphysin (AMPH) from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C185530-100
100 µl (0.9 mg/ml)
$335

Popular AMPH / Amphiphysin Products

Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3757 concentration 10 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2760 concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: Western blot, ELISA
Anti-Amphiphysin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7822 dilution 1:200.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Antibody
Species: Human, Mouse, Rat
Applications: IHC, ICC, Western blot, Immunoprecipitation

Product Description

AMPH / Amphiphysin Antibody (aa1-194) for IHC, WB/Western LS-C185530

Specifications

Target
Human AMPH / Amphiphysin
Synonyms
AMPH, AMPH1, Amphiphysin, Amphiphysin I
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 194 of Amphiphysin (SwissProt P49418).
Epitope
aa1-194
Specificity
Human Amphiphysin
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
About AMPH / Amphiphysin
AMPH / Amphiphysin is a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11. P49418 NM_001635 NP_001626.1


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Images

Immunohistochemistry

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.

Immunohistochemistry

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Amphiphysin antibody [N1N2], N-term dilution:1:500.
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Amphiphysin antibody [N1N2], N-term dilution:1:500.

Immunohistochemistry

IHC of paraffin-embedded NCIN87 xenograft using Amphiphysin antibody at 1:100 dilution.
IHC of paraffin-embedded NCIN87 xenograft using Amphiphysin antibody at 1:100 dilution.

Immunohistochemistry

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.

Western blot

Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 5 % SDS-PAGE. Amphiphysin antibody [N1N2], N-term dilution:1:500
Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 5 % SDS-PAGE. Amphiphysin antibody [N1N2], N-term dilution:1:500

Immunohistochemistry

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.

Immunohistochemistry

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Amphiphysin antibody [N1N2], N-term dilution:1:500.
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Amphiphysin antibody [N1N2], N-term dilution:1:500.

Immunohistochemistry

IHC of paraffin-embedded NCIN87 xenograft using Amphiphysin antibody at 1:100 dilution.
IHC of paraffin-embedded NCIN87 xenograft using Amphiphysin antibody at 1:100 dilution.

Immunohistochemistry

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.

Western blot

Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 5 % SDS-PAGE. Amphiphysin antibody [N1N2], N-term dilution:1:500
Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 5 % SDS-PAGE. Amphiphysin antibody [N1N2], N-term dilution:1:500

Immunohistochemistry

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.

Immunohistochemistry

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Amphiphysin antibody [N1N2], N-term dilution:1:500.
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Amphiphysin antibody [N1N2], N-term dilution:1:500.

Immunohistochemistry

IHC of paraffin-embedded NCIN87 xenograft using Amphiphysin antibody at 1:100 dilution.
IHC of paraffin-embedded NCIN87 xenograft using Amphiphysin antibody at 1:100 dilution.

Immunohistochemistry

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.

Western blot

Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 5 % SDS-PAGE. Amphiphysin antibody [N1N2], N-term dilution:1:500
Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 5 % SDS-PAGE. Amphiphysin antibody [N1N2], N-term dilution:1:500

Immunohistochemistry

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.

Immunohistochemistry

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Amphiphysin antibody [N1N2], N-term dilution:1:500.
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Amphiphysin antibody [N1N2], N-term dilution:1:500.

Immunohistochemistry

IHC of paraffin-embedded NCIN87 xenograft using Amphiphysin antibody at 1:100 dilution.
IHC of paraffin-embedded NCIN87 xenograft using Amphiphysin antibody at 1:100 dilution.

Immunohistochemistry

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Amphiphysin antibody [N1N2], N-term dilution:1:500.

Western blot

Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 5 % SDS-PAGE. Amphiphysin antibody [N1N2], N-term dilution:1:500
Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 5 % SDS-PAGE. Amphiphysin antibody [N1N2], N-term dilution:1:500

Requested From: United States
Date Requested: 10/21/2018

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