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Catalog Number Size Price
LS-C331245-20 20 µl $263 
LS-C331245-50 50 µl $301 
LS-C331245-100 100 µl $359 
LS-C331245-200 200 µl $473 
ALPL / Alkaline Phosphatase Antibody - Immunohistochemistry of paraffin-embedded mouse brain using ALPL antibodyat dilution of 1:100 (40x lens).
ALPL / Alkaline Phosphatase Antibody - Western blot analysis of HepG2 cell and Hela cell lysate.
ALPL / Alkaline Phosphatase Antibody - Western blot analysis of extracts of HeLa cells, using ALPL antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.
ALPL / Alkaline Phosphatase Antibody - Immunohistochemistry of paraffin-embedded mouse brain using ALPL antibodyat dilution of 1:100 (40x lens).
ALPL / Alkaline Phosphatase Antibody - Western blot analysis of HepG2 cell and Hela cell lysate.
ALPL / Alkaline Phosphatase Antibody - Western blot analysis of extracts of HeLa cells, using ALPL antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.
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Polyclonal Rabbit anti‑Human ALPL / Alkaline Phosphatase Antibody (IHC, WB) LS‑C331245

Polyclonal Rabbit anti‑Human ALPL / Alkaline Phosphatase Antibody (IHC, WB) LS‑C331245

Antibody:
ALPL / Alkaline Phosphatase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$263
LS-C331245-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ALPL / Alkaline Phosphatase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Alkaline Phosphatase antibody LS-C331245 is an unconjugated rabbit polyclonal antibody to Alkaline Phosphatase (ALPL) from human. It is reactive with human and mouse. Validated for IHC and WB.
Target
Human ALPL / Alkaline Phosphatase
Synonyms
ALPL | Alkaline phosphomonoesterase | AP-TNAP | Alkaline phosphatase liver | APTNAP | Glycerophosphatase | Tissue-nonspecific ALP | TNAP | HOPS | Liver alkaline phosphatase | TNSALP
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-270 of human ALPL (NP_000469.3). PEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIW
Specificity
Human ALPL / Alkaline Phosphatase
Applications
  • IHC (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 48kDa/51kDa/57kDa, while the observed MW by Western blot was 88kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ALPL / Alkaline Phosphatase
P05186 NM_000478 NP_000469.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/2/2022