Human ALPL / Alkaline Phosphatase

Mouse

Human, Bovine (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated. Also available conjugated with Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Purified alkaline phosphatase (native) from human bone.

Recognizes Alkaline Phosphatase from human bone. Species cross-reactivity: Alkaline phosphatase from human placenta and calf intestine.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot
• ELISA

Immunohistochemistry: LS-B3666 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3666 was determined to be 10 ug/ml. Suitable for use in ELISA and Western Blot. Western Blot: Stains a band of bone AP at ~90kD.

PBS, pH 7.2, 0.09% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05186 NCBI: NM_000478 NP_000469.3