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Catalog Number Size Price
LS-C742445-100 100 µg (0.5 mg/ml) $503 
ALPL / Alkaline Phosphatase Antibody - Goat anti-ALPL (aa42-53) Antibody (0.1µg/ml) staining of Human Kidney (A), Lung (B) and Adrenal Gland (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.

Polyclonal Goat anti‑Human ALPL / Alkaline Phosphatase Antibody (aa42‑53, WB) LS‑C742445

Polyclonal Goat anti‑Human ALPL / Alkaline Phosphatase Antibody (aa42‑53, WB) LS‑C742445

Antibody:
ALPL / Alkaline Phosphatase Goat anti-Human Polyclonal (aa42-53) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C742445-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ALPL / Alkaline Phosphatase Goat anti-Human Polyclonal (aa42-53) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Alkaline Phosphatase antibody LS-C742445 is an unconjugated goat polyclonal antibody to human Alkaline Phosphatase (ALPL) (aa42-53). Validated for Peptide-ELISA and WB.
Target
Human ALPL / Alkaline Phosphatase
Synonyms
ALPL | Alkaline phosphomonoesterase | AP-TNAP | Alkaline phosphatase liver | APTNAP | Glycerophosphatase | Tissue-nonspecific ALP | TNAP | HOPS | Liver alkaline phosphatase | TNSALP
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-ELQKLNTNVAKN, from the internal region (near N-Terminus) of the protein sequence according to NP_000469.3.
Epitope
aa42-53
Specificity
This antibody is expected to recognize isoform 1 (NP_000469.3) only. The immunizing peptide represents the N terminus of the mature protein.
Applications
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 55kDa band observed in Human Kidney, Lung and Adrenal Gland lysates (calculated MW of 57.3kDa according to NP_000469.3). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ALPL / Alkaline Phosphatase
P05186 NM_000478 NP_000469.3

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/23/2024