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Catalog Number Size Price
LS-C744967-100 100 µg (1 mg/ml) $485 
ALPI / Alkaline Phosphatase Antibody - Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (611-1302) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.
ALPI / Alkaline Phosphatase Antibody - Western Blot of rabbit Anti-Alkaline Phosphatase Primary Antibody. Lane 1: Alkaline Phosphatase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Alkaline Phosphatase primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 58 kDa, 58 kDa for Alkaline Phosphatase. Other band(s): None.
ALPI / Alkaline Phosphatase Antibody - Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (611-1302) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.
ALPI / Alkaline Phosphatase Antibody - Western Blot of rabbit Anti-Alkaline Phosphatase Primary Antibody. Lane 1: Alkaline Phosphatase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Alkaline Phosphatase primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 58 kDa, 58 kDa for Alkaline Phosphatase. Other band(s): None.
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Polyclonal Rabbit anti‑Human ALPI / Alkaline Phosphatase Antibody (Lyophilized, WB) LS‑C744967

Polyclonal Rabbit anti‑Human ALPI / Alkaline Phosphatase Antibody (Lyophilized, WB) LS‑C744967

Antibody:
ALPI / Alkaline Phosphatase Rabbit anti-Human Polyclonal (Lyophilized) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Lyophilized
Price
Catalog Number
$485
LS-C744967-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
ALPI / Alkaline Phosphatase Rabbit anti-Human Polyclonal (Lyophilized) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Lyophilized

Specifications

Description
Alkaline Phosphatase antibody LS-C744967 is an unconjugated rabbit polyclonal antibody to human Alkaline Phosphatase (ALPI). Validated for ELISA and WB.
Target
Human ALPI / Alkaline Phosphatase
Synonyms
ALPI | Alkaline phosphomonoesterase | Kasahara isozyme | Glycerophosphatase | Alkaline Phosphatase | IAP
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Alkaline Phosphatase [human intestine].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Human Intestine]. Cross reactivity against Alkaline Phosphatase from other sources is unknown.
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:4000 - 1:20000)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ALPI / Alkaline Phosphatase
P09923 NM_001631 NP_001622.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021