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Catalog Number Size Price
LS-C745089-25 25 µl (1 mg/ml) $304 
ALPI / Alkaline Phosphatase Antibody - Western Blot of rabbit anti-Alkaline Phosphatase (E. Coli) Antibody Peroxidase Conjugated. Lane 1: Alkaline Phosphatase (E. Coli). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Alkaline Phosphatase (E. Coli).

Polyclonal Rabbit anti‑E. coli ALPI / Alkaline Phosphatase Antibody (Liquid, HRP, WB) LS‑C745089

Polyclonal Rabbit anti‑E. coli ALPI / Alkaline Phosphatase Antibody (Liquid, HRP, WB) LS‑C745089

Antibody:
ALPI / Alkaline Phosphatase Rabbit anti-E. coli Polyclonal (Liquid) (HRP) Antibody
Application:
WB, ELISA
Reactivity:
E. coli
Format:
Horseradish Peroxidase, Liquid
Price
Catalog Number
$304
LS-C745089-25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ALPI / Alkaline Phosphatase Rabbit anti-E. coli Polyclonal (Liquid) (HRP) Antibody
Application:
WB, ELISA
Reactivity:
E. coli
Format:
Horseradish Peroxidase, Liquid

Specifications

Description
Alkaline Phosphatase antibody LS-C745089 is an HRP-conjugated rabbit polyclonal antibody to e. coli Alkaline Phosphatase (ALPI). Validated for ELISA and WB.
Target
E. coli ALPI / Alkaline Phosphatase
Synonyms
ALPI | Alkaline phosphomonoesterase | Kasahara isozyme | Glycerophosphatase | Alkaline Phosphatase | IAP
Host
Rabbit
Reactivity
E. coli (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
Alkaline Phosphatase [E.coli]
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli]. No cross reactivity occurs against mammalian forms of Alkaline Phosphatase.
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:4000 - 1:20000)
Usage
Applications should be user optimized.
Presentation
10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ALPI / Alkaline Phosphatase
P09923 NM_001631 NP_001622.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/19/2024