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Catalog Number Size Price
LS-C745056-25 25 µl (1 mg/ml) $275 
ALPI / Alkaline Phosphatase Antibody - Western Blot of rabbit anti-Alkaline Phosphatase (Calf Intestine) Antibody. Lane 1: Alkaline Phosphatase (Calf Intestine). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (Calf Intestine) Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 57 kDa, 60 kDa for Alkaline Phosphatase (Calf Intestine).
ALPI / Alkaline Phosphatase Antibody - Western Blot of rabbit anti Alkaline Phosphatase antibody Lane 1: Calf intestine Alkaline Phosphatase. Load: ~1.5 µg Block: 5% Blotto 1 hour at 4°C. Primary antibody: diluted to 1:1000 in 5% Blotto overnight at 4°C. Secondary antibody: Goat anti Rabbit 1:20,000 in MB-070 1 Hour 4°C Substrate: Femtomax 110
ALPI / Alkaline Phosphatase Antibody - Western Blot of rabbit anti-Alkaline Phosphatase (Calf Intestine) Antibody. Lane 1: Alkaline Phosphatase (Calf Intestine). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (Calf Intestine) Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 57 kDa, 60 kDa for Alkaline Phosphatase (Calf Intestine).
ALPI / Alkaline Phosphatase Antibody - Western Blot of rabbit anti Alkaline Phosphatase antibody Lane 1: Calf intestine Alkaline Phosphatase. Load: ~1.5 µg Block: 5% Blotto 1 hour at 4°C. Primary antibody: diluted to 1:1000 in 5% Blotto overnight at 4°C. Secondary antibody: Goat anti Rabbit 1:20,000 in MB-070 1 Hour 4°C Substrate: Femtomax 110
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Polyclonal Rabbit anti‑Bovine ALPI / Alkaline Phosphatase Antibody (Liquid, IHC, WB) LS‑C745056

Polyclonal Rabbit anti‑Bovine ALPI / Alkaline Phosphatase Antibody (Liquid, IHC, WB) LS‑C745056

Antibody:
ALPI / Alkaline Phosphatase Rabbit anti-Bovine Polyclonal (Liquid) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Liquid
Price
Catalog Number
$275
LS-C745056-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
ALPI / Alkaline Phosphatase Rabbit anti-Bovine Polyclonal (Liquid) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Liquid

Specifications

Description
Alkaline Phosphatase antibody LS-C745056 is an unconjugated rabbit polyclonal antibody to bovine Alkaline Phosphatase (ALPI). Validated for ELISA, IHC and WB.
Target
Bovine ALPI / Alkaline Phosphatase
Synonyms
ALPI | Alkaline phosphomonoesterase | Kasahara isozyme | Glycerophosphatase | Alkaline Phosphatase | IAP
Host
Rabbit
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
Alkaline Phosphatase [Calf intestine].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:100000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ALPI / Alkaline Phosphatase
P09923 NM_001631 NP_001622.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021