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| Catalog Number | Size | Price |
|---|---|---|
| LS-C39983-500 | 500 µl | $858 |
Monoclonal Mouse anti‑Chicken Alpha Tubulin Antibody (aa426‑450, IHC, IF, WB) LS‑C39983
Monoclonal Mouse anti‑Chicken Alpha Tubulin Antibody (aa426‑450, IHC, IF, WB) LS‑C39983
Antibody:
Alpha Tubulin Mouse anti-Chicken Monoclonal (aa426-450) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP
Reactivity:
Chicken, Human, Mouse, Rat, Bovine, Guinea pig, Pig, Frog
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Alpha Tubulin Mouse anti-Chicken Monoclonal (aa426-450) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP
Reactivity:
Chicken, Human, Mouse, Rat, Bovine, Guinea pig, Pig, Frog
Format:
Unconjugated, Unmodified
Specifications
Description
Alpha Tubulin antibody LS-C39983 is an unconjugated mouse monoclonal antibody to Alpha Tubulin (aa426-450) from chicken. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, IP and WB.
Host
Mouse
Reactivity
Chicken, Human, Mouse, Rat, Bovine, Guinea pig, Pig, Frog
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Native chick brain microtubules. Epitope: Localizes between aa 426-450. aa 426-430 are critical for reactivity (2). Cellular Localization: Cytoplasmic.
Epitope
aa426-450
Specificity
Recognizes chicken tubulin alpha (MW 57kD). Causes the 10nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. Does not block microtubule assembly. Does not inhibit polymerization or depolymerization of platelet tubulin in vitro. Blocks (70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. Species cross-reactivity: bovine, human, porcine, rat, mouse, guinea pig, gerbil and frog.
Applications
- IHC
- IHC - Paraffin
- IHC - Frozen
- Immunofluorescence
- Western blot (1 - 2 µg/ml)
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Electron Microscopy, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Western Blot: 1-2 ug/ml for 2hrs at RT. Immunohistochemistry (Frozen and formalin/paraffin): 1:200 for 20 min at RT. Immunoprecipitation (Native and denatured): 2 ug/mg protein lysate using Protein G. Positive control: LS174T, MAD109 cells. Skin or lung.
Presentation
PBS, pH 7.4, 15 mM Sodium Azide, 0.2% BSA
Restrictions
For research use only. Intended for use by laboratory professionals.
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Customer Reviews (0)
Featured Products
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Guinea pig, Hamster, Pig, Yeast, Amphibian, Kangaroo, Sea urchin
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat, Bovine, Chicken
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Pig
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation, ELISA
Species:
Human, Mouse, Rat, Bovine, Goat, Sheep, Chicken
Applications:
IHC, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
