Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C744940-50 50 µg (1 mg/ml) $492 
AKT3 Antibody - Immunohistochemistry of Mouse Anti-AKT3 antibody. Tissue: human prostate carcinoma. A) AKT-3 antibody produced using CELLine, B) AKT-3 antibody produced using roller bottle. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT-3 unconjugated antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 1 h at RT. Localization: AKT3 is nuclear and occasionally cytoplasmic. Staining: AKT3 as precipitated brown signal with hematoxylin purple nuclear counterstain.
AKT3 Antibody - Immunohistochemistry of Mouse Monoclonal anti AKT3 unconjugated Antibody in Mouse Embryonic Kidney. Tissue: Mouse Liver. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker. Primary antibody: 2ug/ml 1.5 hour @ room T. Secondary Ab: MOUSE ON MOUSE HRP POLYMER 45” RT.
AKT3 Antibody - Western Blot of Mouse anti-AKT3 antibody. Lane 1: Control. Lane 2: Rapa. Lane 3: T50. Lane 4: T250. Lane 5: Control. Lane 6: Rapa. Lane 7: T50. Lane 8: T250. Lane 9: AKT3 null. Load: 35 µg per lane. Primary antibody: AKT-3 unconjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: Anti mouse secondary antibody at 1:20,000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT3.
AKT3 Antibody - Western Blot of Mouse Anti-AKT3 antibody. Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 µg per lane. Primary antibody: AKT-3 unconjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: Anti mouse secondary antibody at 1:20,000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT3.
AKT3 Antibody - Western Blot of Mouse anti-AKT3 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT3 unconjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT3. Other band(s): none.
AKT3 Antibody - Immunohistochemistry of Mouse Anti-AKT3 antibody. Tissue: human prostate carcinoma. A) AKT-3 antibody produced using CELLine, B) AKT-3 antibody produced using roller bottle. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT-3 unconjugated antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 1 h at RT. Localization: AKT3 is nuclear and occasionally cytoplasmic. Staining: AKT3 as precipitated brown signal with hematoxylin purple nuclear counterstain.
AKT3 Antibody - Immunohistochemistry of Mouse Monoclonal anti AKT3 unconjugated Antibody in Mouse Embryonic Kidney. Tissue: Mouse Liver. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker. Primary antibody: 2ug/ml 1.5 hour @ room T. Secondary Ab: MOUSE ON MOUSE HRP POLYMER 45” RT.
AKT3 Antibody - Western Blot of Mouse anti-AKT3 antibody. Lane 1: Control. Lane 2: Rapa. Lane 3: T50. Lane 4: T250. Lane 5: Control. Lane 6: Rapa. Lane 7: T50. Lane 8: T250. Lane 9: AKT3 null. Load: 35 µg per lane. Primary antibody: AKT-3 unconjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: Anti mouse secondary antibody at 1:20,000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT3.
AKT3 Antibody - Western Blot of Mouse Anti-AKT3 antibody. Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 µg per lane. Primary antibody: AKT-3 unconjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: Anti mouse secondary antibody at 1:20,000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT3.
AKT3 Antibody - Western Blot of Mouse anti-AKT3 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT3 unconjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT3. Other band(s): none.
1 of 5
2 of 5
3 of 5
4 of 5
5 of 5

Monoclonal Mouse anti‑Human AKT3 Antibody (clone 25F6.F6.D8, RPE, Internal, IHC, IF, WB) LS‑C744940

Monoclonal Mouse anti‑Human AKT3 Antibody (clone 25F6.F6.D8, RPE, Internal, IHC, IF, WB) LS‑C744940

Antibody:
AKT3 Mouse anti-Human Monoclonal (Internal) (RPE) (25F6.F6.D8) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
R. Phycoerythrin, Unmodified
Price
Catalog Number
$492
LS-C744940-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
AKT3 Mouse anti-Human Monoclonal (Internal) (RPE) (25F6.F6.D8) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
R. Phycoerythrin, Unmodified

Specifications

Description
AKT3 antibody LS-C744940 is an RPE-conjugated mouse monoclonal antibody to AKT3 (Internal) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IF, IHC and WB.
Target
Human AKT3
Synonyms
AKT3 | AKT3/PKBgamma | PRKBG | PKB gamma | Protein kinase Akt-3 | RAC-gamma | MPPH | PKB-GAMMA | PKBG | Protein kinase B gamma | RAC-PK-gamma | STK-2
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
25F6.F6.D8
Conjugations
R. Phycoerythrin
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Anti-AKT3 Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography using a synthetic peptide corresponding to internal residues of human AKT3 protein.
Epitope
Internal
Specificity
The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from tissue culture by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.
Applications
  • IHC
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl
Reconstitution
Reconstitute in 50µL of deionized water.
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT3
Q9Y243 NM_005465 NP_005456.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021