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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AKT2 Antibody (phospho‑Ser474) LS‑C416640
AKT2 antibody LS-C416640 is an unconjugated rabbit polyclonal antibody to AKT2 from human, mouse and rat. Validated for IHC and WB.
Catalog
Size
Price
LS-C416640-100
100 µg (1 mg/ml)
$305
Description
AKT2 antibody LS-C416640 is an unconjugated rabbit polyclonal antibody to AKT2 from human, mouse and rat. Validated for IHC and WB.
Target
Human AKT2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
AKT2 antibody was raised against synthetic peptide from human Akt2 around the phosphorylation site of Serine 474.
Epitope
pSer474
Specificity
Phospho-Akt2 (Ser474) Antibody detects endogenous levels of Akt2 only when phosphorylated at Serine 474.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
About AKT2
P31751 NM_001626 NP_001617.1

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Images

Western blot

Western blot of Akt2 phosphorylation expression in TNF- alpha treated A2780 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot of Akt2 phosphorylation expression in TNF- alpha treated A2780 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Western blot

Western blot of Akt2 phosphorylation expression in TNF- alpha treated A2780 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot of Akt2 phosphorylation expression in TNF- alpha treated A2780 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Western blot

Western blot of Akt2 phosphorylation expression in TNF- alpha treated A2780 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot of Akt2 phosphorylation expression in TNF- alpha treated A2780 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Western blot

Western blot of Akt2 phosphorylation expression in TNF- alpha treated A2780 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot of Akt2 phosphorylation expression in TNF- alpha treated A2780 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Requested From: United States
Date Requested: 12/11/2018
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