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| Catalog Number | Size | Price |
|---|---|---|
| LS-C744918-50 | 50 µg (1 mg/ml) | $582 |
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Monoclonal Mouse anti‑Human AKT1 Antibody (clone 5E5.F5.D7, FITC, Internal, IHC, IF, WB) LS‑C744918
Monoclonal Mouse anti‑Human AKT1 Antibody (clone 5E5.F5.D7, FITC, Internal, IHC, IF, WB) LS‑C744918
Antibody:
AKT1 Mouse anti-Human Monoclonal (Internal) (FITC) (5E5.F5.D7) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
FITC, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
AKT1 Mouse anti-Human Monoclonal (Internal) (FITC) (5E5.F5.D7) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
FITC, Unmodified
Specifications
Description
AKT1 antibody LS-C744918 is an FITC-conjugated mouse monoclonal antibody to AKT1 (Internal) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IF, IHC and WB.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Mouse
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Clone
5E5.F5.D7
Conjugations
FITC
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein followed by monoclonal development.
Epitope
Internal
Specificity
The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
Applications
- IHC
- Immunofluorescence
- Western blot
- Flow Cytometry
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 50µL of deionized water.
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About AKT1
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
