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Antibodies > AKT1 > LS-C744900
 
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Catalog Number Size Price
LS-C744900-25 25 µl (1 mg/ml) $304 
AKT1 Antibody - Western Blot of Mouse anti-AKT1 antibody. Lane 1: AKT1 Null. Lane 2: WT. Lane 3: MEF #1. Lane 4 : A549. Lane 5: Calu-1. Lane 6: PC-3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Lane 11: C2C12. Load: 20 ug per lane. Primary antibody: AKT1 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 56 kDa for AKT1. Other band(s): none.
AKT1 Antibody - Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT2. Other band(s): none.
AKT1 Antibody - Flow Cytometry of Mouse anti-AKT1 antibody. Cells: LNCap Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT1 antibody at 1.0 µg/mL for 20 min at 4°C.
AKT1 Antibody - Plate was coated with monoclonal anti AKT1 antibody followed by incubation with recombinant AKT1, AKT2, AKT3 proteins. Binding was detected with biotinylated monoclonal anti AKT pS473. The signal shows specificity of the monoclonal anti-AKT1 antibody to recombinant isoform AKT1 protein and not the isoform 2 and 3
AKT1 Antibody - Western Blot of Mouse anti-AKT1 antibody. Lane 1: AKT1 Null. Lane 2: WT. Lane 3: MEF #1. Lane 4 : A549. Lane 5: Calu-1. Lane 6: PC-3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Lane 11: C2C12. Load: 20 ug per lane. Primary antibody: AKT1 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 56 kDa for AKT1. Other band(s): none.
AKT1 Antibody - Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT2. Other band(s): none.
AKT1 Antibody - Flow Cytometry of Mouse anti-AKT1 antibody. Cells: LNCap Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT1 antibody at 1.0 µg/mL for 20 min at 4°C.
AKT1 Antibody - Plate was coated with monoclonal anti AKT1 antibody followed by incubation with recombinant AKT1, AKT2, AKT3 proteins. Binding was detected with biotinylated monoclonal anti AKT pS473. The signal shows specificity of the monoclonal anti-AKT1 antibody to recombinant isoform AKT1 protein and not the isoform 2 and 3
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Monoclonal Mouse anti‑Human AKT1 Antibody (clone 14E5.A2.B2.H9, Internal, IHC, WB) LS‑C744900

Monoclonal Mouse anti‑Human AKT1 Antibody (clone 14E5.A2.B2.H9, Internal, IHC, WB) LS‑C744900

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Antibody:
AKT1 Mouse anti-Human Monoclonal (Internal) (14E5.A2.B2.H9) Antibody
Application:
IHC, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$304
LS-C744900-25
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
AKT1 Mouse anti-Human Monoclonal (Internal) (14E5.A2.B2.H9) Antibody
Application:
IHC, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
AKT1 antibody LS-C744900 is an unconjugated mouse monoclonal antibody to human AKT1 (Internal). Validated for ELISA, Flow, IHC and WB.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
14E5.A2.B2.H9
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Anti-AKT1 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein.
Epitope
Internal
Specificity
The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
Applications
  • IHC (20 µg/ml)
  • Western blot (1:1000)
  • Flow Cytometry
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT1
P31749 NM_005163 NP_005154.2

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AKT1 Antibody - Anti-AKT Monoclonal Antibody - Western Blot. anti-AKT Monoclonal Antibody in western blot shows detection of AKT (indicated by arrowhead at ~56 kD) on unstimulated (lane 1) and PDGF stimulated NIH/3T3 cell lysates (lane 2). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO (B501-0500 in TBS for 2h at RT. The membrane was probed with Anti-AKT antibody at a 1:1000 dilution in TBS with 5% BLOTTO, overnight at 4° C. For detection HRP conjugated Gt-a-Mouse IgG (p/n LS-C60680) was used at a 1:40000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).
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Species: Human, Mouse
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Species: Human
Applications: Western blot, ELISA
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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