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Catalog Number Size Price
LS-C744832-1 1 mg $1,934 
AKT1 Antibody - Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. Lane 2: PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.

Monoclonal Mouse anti‑Human AKT1 Antibody (C‑Terminus, IHC, WB) LS‑C744832

Monoclonal Mouse anti‑Human AKT1 Antibody (C‑Terminus, IHC, WB) LS‑C744832

Antibody:
AKT1 Mouse anti-Human Monoclonal (C-Terminus) Antibody
Application:
IHC, WB, Flo, ELISA
Reactivity:
Human, Chimpanzee, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$1,934
LS-C744832-1
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
AKT1 Mouse anti-Human Monoclonal (C-Terminus) Antibody
Application:
IHC, WB, Flo, ELISA
Reactivity:
Human, Chimpanzee, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
AKT1 antibody LS-C744832 is an unconjugated mouse monoclonal antibody to AKT1 (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IHC and WB.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Mouse
Reactivity
Human, Chimpanzee, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Anti-Akt monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human AKT1 protein.
Epitope
C-Terminus
Specificity
This antibody is specific for human and mouse AKT protein. A BLAST analysis was used to suggest cross-reactivity with AKT from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined.  Cross-reactivity with AKT2 and AKT3 has not been determined.
Applications
  • IHC (20 µg/ml)
  • Western blot (1:500 - 1:3000)
  • Flow Cytometry
  • ELISA (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT1
P31749 NM_005163 NP_005154.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/17/2021