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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AKT1 + AKT2 Antibody (aa71‑91) LS‑C387957
AKT1 + AKT2 antibody LS-C387957 is an unconjugated rabbit polyclonal antibody to AKT1 + AKT2 from human, mouse, bovine and other species. Validated for WB.
Catalog
Size
Price
LS-C387957-100
100 µg (0.5 mg/ml)
$415
Description
AKT1 + AKT2 antibody LS-C387957 is an unconjugated rabbit polyclonal antibody to AKT1 + AKT2 from human, mouse, bovine and other species. Validated for WB.
Target
Human AKT1 + AKT2
Host
Rabbit
Reactivity
Human, Mouse, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Sheep, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 1 µg/ml)
Immunogen
AKT1 + AKT2 antibody was raised against synthetic peptide from the N-terminus of human AKT1/2 (NTFIIRCLQWTTVIERTFHVE, aa71-91 of UniProt P31749). Percent identity by BLAST analysis: Human, Mouse, Ferret, Sheep, Bovine, Goat, Hamster, Elephant, Horse, Pig, Guinea pig, Turkey, Chicken, Platypus, Lizard, Trout, Medaka, Catfish, Zebrafish, Stickleback, Dog (100%); Pufferfish (95%).
Epitope
aa71-91
Specificity
Human AKT1 + AKT2
Usage
AKT protein family, which members are also called protein kinases B (PKB) plays an important role in mammalian cellular signaling. In humans, there are three genes in the Akt family: Akt1, Akt2, and Akt3. These genes code for enzymes that are members of the serine/threonine-specific protein kinase family. Akt1 is involved in cellular survival pathways, by inhibiting apoptotic processes. Akt1 is also able to induce protein synthesis pathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. Since it can block apoptosis, and thereby promote cell survival, Akt1 has been implicated as a major factor in many types of cancer. Akt (now also called Akt1) was originally identified as the oncogene in the transforming retrovirus, AKT8. AKT8 was isolated by Stephen Staal in the laboratory of Wallace P. Rowe. he subsequently cloned v-akt and human AKT1 and AKT2 while on staff at the Johns Hopkins Oncology Center. Akt2 is an important signaling molecule in the Insulin signaling pathway, it is required to induce glucose transport. Franke et al. (1995) show that AKT1 and AKT2 are activated by PDGF. The activation was rapid and specific, and it was abrogated by mutations in the Akt Pleckstrin homology (PH) domain. They identify that Akt is a novel target of PI 3-kinase and suggest that the Akt PH domain may be a mediator of PI 3-kinase signaling.
Presentation
Lyophilized from PBS, 2.5% BSA, 0.025% sodium azide/thimerosal.
Reconstitution
0.2 ml Sterile distilled water
Storage
Prior to reconstitution, 4°C. Following reconstitution store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

Popular AKT1 + AKT2 Products

Anti-AKT antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Akt (Phospho-Thr308) Antibody. The picture on the right is blocked with the phospho peptide.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Western blot analysis of AKT1/2 (pT308/309) expression in HeLa (A); PC3 (B) whole cell lysates.
Species: Human, Mouse, Rat, Bovine
Applications: Western blot
Antibody
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Mink, Pig, Chicken, Xenopus, Fish, Zebrafish, Drosophila, Yeast, C. elegans
Applications: Flow Cytometry

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Images

Western blot

Western blot testing of AKT1/2 antibody and Lane 1: MCF-7; 2: HeLa; 3: MM453; 4: HT1080; 5: COLO320 cell lysate
Western blot testing of AKT1/2 antibody and Lane 1: MCF-7; 2: HeLa; 3: MM453; 4: HT1080; 5: COLO320 cell lysate

Western blot

Western blot testing of AKT1/2 antibody and Lane 1: MCF-7; 2: HeLa; 3: MM453; 4: HT1080; 5: COLO320 cell lysate
Western blot testing of AKT1/2 antibody and Lane 1: MCF-7; 2: HeLa; 3: MM453; 4: HT1080; 5: COLO320 cell lysate

Western blot

Western blot testing of AKT1/2 antibody and Lane 1: MCF-7; 2: HeLa; 3: MM453; 4: HT1080; 5: COLO320 cell lysate
Western blot testing of AKT1/2 antibody and Lane 1: MCF-7; 2: HeLa; 3: MM453; 4: HT1080; 5: COLO320 cell lysate

Western blot

Western blot testing of AKT1/2 antibody and Lane 1: MCF-7; 2: HeLa; 3: MM453; 4: HT1080; 5: COLO320 cell lysate
Western blot testing of AKT1/2 antibody and Lane 1: MCF-7; 2: HeLa; 3: MM453; 4: HT1080; 5: COLO320 cell lysate

Requested From: United States
Date Requested: 12/19/2018
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