Human, Mouse, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Sheep, Chicken, Zebrafish
(tested or 100% immunogen sequence identity)
- Western blot (0.5 - 1 µg/ml)
AKT1 + AKT2 antibody was raised against synthetic peptide from the N-terminus of human AKT1/2 (NTFIIRCLQWTTVIERTFHVE, aa71-91 of UniProt P31749). Percent identity by BLAST analysis: Human, Mouse, Ferret, Sheep, Bovine, Goat, Hamster, Elephant, Horse, Pig, Guinea pig, Turkey, Chicken, Platypus, Lizard, Trout, Medaka, Catfish, Zebrafish, Stickleback, Dog (100%); Pufferfish (95%).
Human AKT1 + AKT2
AKT protein family, which members are also called protein kinases B (PKB) plays an important role in mammalian cellular signaling. In humans, there are three genes in the Akt family: Akt1, Akt2, and Akt3. These genes code for enzymes that are members of the serine/threonine-specific protein kinase family. Akt1 is involved in cellular survival pathways, by inhibiting apoptotic processes. Akt1 is also able to induce protein synthesis pathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. Since it can block apoptosis, and thereby promote cell survival, Akt1 has been implicated as a major factor in many types of cancer. Akt (now also called Akt1) was originally identified as the oncogene in the transforming retrovirus, AKT8. AKT8 was isolated by Stephen Staal in the laboratory of Wallace P. Rowe. he subsequently cloned v-akt and human AKT1 and AKT2 while on staff at the Johns Hopkins Oncology Center. Akt2 is an important signaling molecule in the Insulin signaling pathway, it is required to induce glucose transport. Franke et al. (1995) show that AKT1 and AKT2 are activated by PDGF. The activation was rapid and specific, and it was abrogated by mutations in the Akt Pleckstrin homology (PH) domain. They identify that Akt is a novel target of PI 3-kinase and suggest that the Akt PH domain may be a mediator of PI 3-kinase signaling.
Lyophilized from PBS, 2.5% BSA, 0.025% sodium azide/thimerosal.
0.2 ml Sterile distilled water
Prior to reconstitution, 4°C. Following reconstitution store at -20°C. Avoid freeze-thaw cycles.
For research use only.
About AKT1 + AKT2
AKT, also known as protein kinase B (PKB), constitutes a family of serine/threonine kinases. AKT has 3 isoforms: AKT1, AKT2), and AKT3). These isoforms share a high degree of homology, especially in the catalytic domains (87%-90% identical amino acids).