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Catalog Number Size Price
LS-C387957-100 100 µg $506 
AKT1 + AKT2 Antibody - Western blot testing of AKT1/2 antibody and Lane 1: MCF-7; 2: HeLa; 3: MM453; 4: HT1080; 5: COLO320 cell lysate

Polyclonal Rabbit anti‑Human AKT1 + AKT2 Antibody (aa71‑91, WB) LS‑C387957

Polyclonal Rabbit anti‑Human AKT1 + AKT2 Antibody (aa71‑91, WB) LS‑C387957

Antibody:
AKT1 + AKT2 Rabbit anti-Human Polyclonal (aa71-91) Antibody
Application:
WB
Reactivity:
Human, Mouse, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Sheep, Chicken, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$506
LS-C387957-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
AKT1 + AKT2 Rabbit anti-Human Polyclonal (aa71-91) Antibody
Application:
WB
Reactivity:
Human, Mouse, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Sheep, Chicken, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
AKT1 + AKT2 antibody LS-C387957 is an unconjugated rabbit polyclonal antibody to AKT1 + AKT2 (aa71-91) from human. It is reactive with human, mouse, bovine and other species. Validated for WB.
Target
Human AKT1 + AKT2
Synonyms
AKT1/AKT2
Host
Rabbit
Reactivity
Human, Mouse, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Sheep, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the N-Terminus of human AKT1/2 (NTFIIRCLQWTTVIERTFHVE, aa71-91 of UniProt P31749). Percent identity by BLAST analysis: Human, Mouse, Ferret, Sheep, Bovine, Goat, Hamster, Elephant, Horse, Pig, Guinea pig, Turkey, Chicken, Platypus, Lizard, Trout, Medaka, Catfish, Zebrafish, Stickleback, Dog (100%); Pufferfish (95%).
Epitope
aa71-91
Specificity
Human AKT1 + AKT2
Applications
  • Western blot (0.5 - 1 µg/ml)
Usage
AKT protein family, which members are also called protein kinases B (PKB) plays an important role in mammalian cellular signaling. In humans, there are three genes in the Akt family: Akt1, Akt2, and Akt3. These genes code for enzymes that are members of the serine/threonine-specific protein kinase family. Akt1 is involved in cellular survival pathways, by inhibiting apoptotic processes. Akt1 is also able to induce protein synthesis pathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. Since it can block apoptosis, and thereby promote cell survival, Akt1 has been implicated as a major factor in many types of cancer. Akt (now also called Akt1) was originally identified as the oncogene in the transforming retrovirus, AKT8. AKT8 was isolated by Stephen Staal in the laboratory of Wallace P. Rowe. he subsequently cloned v-akt and human AKT1 and AKT2 while on staff at the Johns Hopkins Oncology Center. Akt2 is an important signaling molecule in the Insulin signaling pathway, it is required to induce glucose transport. Franke et al. (1995) show that AKT1 and AKT2 are activated by PDGF. The activation was rapid and specific, and it was abrogated by mutations in the Akt Pleckstrin homology (PH) domain. They identify that Akt is a novel target of PI 3-kinase and suggest that the Akt PH domain may be a mediator of PI 3-kinase signaling.
Presentation
Lyophilized from PBS, 2.5% BSA, 0.025% sodium azide/thimerosal.
Reconstitution
Reconstitute in sterile distilled water.
Storage
Prior to reconstitution, 4°C. Following reconstitution store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/8/2021