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Antibodies > AHSP / EDRF > LS-C744601
 
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Catalog Number Size Price
LS-C744601-25 25 µl (115 mg/ml) $304 
AHSP / EDRF Antibody - Immunohistochemistry of rabbit anti-AHSP antibody. Tissue: A. Primary myelofibrosis, H&E. B. CD235a stains both nucleated EPs and mature, anucleate RBCs. AHSP C. and CD71 D. variably stain megakaryocytes and also stain nucleated EPs. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max – fully automated for IHC.
AHSP / EDRF Antibody - Immunohistochemistry of rabbit anti-AHSP antibody. Tissue: AHSP A. stains residual EPs and not lymphoma cells in DLBCL, whereas CD71 B. stains both lymphomacells and EPs. C. Corresponding H&E. AHSP D. does not metastatic carcinoma, whereas CD71 E. does. F. Corresponding H&E. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max – fully automated for IHC.
AHSP / EDRF Antibody - Immunohistochemistry of rabbit anti-AHSP antibody. Tissue: Giant pronormoblasts are evident in parvoviral infection (H&E, A). B. CD235a does not stain giant pronormoblasts. AHSP C. and CD71 D. stain giant pronormoblasts. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max – fully automated for IHC.
AHSP / EDRF Antibody - Immunohistochemistry of rabbit anti-AHSP antibody. Tissue: A. Normal bone marrow, H&E. B. CD235a stains both nucleated EPs and mature, anucleate RBCs. C. AHSP stains nucleated EPs, but not mature, anucleate RBCs. D. CD71 stains nucleated EPs, but not mature, anucleate RBCs. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max – fully automated for IHC.
AHSP / EDRF Antibody - Immunohistochemistry of rabbit anti-AHSP antibody. Tissue: A. Acute erythroleukemia, H&E. B. CD235a stains erythroid blasts and mature, anucleate RBCs. C. AHSP stains erythroid blasts. D. CD71 stains erythroid blasts. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max – fully automated for IHC.
AHSP / EDRF Antibody - Immunohistochemistry of rabbit anti-AHSP antibody. Tissue: AHSP stains residual EPs and not myeloid blasts in acute myeloid leukemia with minimal differentiation (A), whereas CD71 stains both myeloid blasts and EPs (B). AHSP does not stain myeloid blasts in acute myelomonocytic leukemia (D), whereas CD71 does (E). C and F are corresponding H&Es, respectively. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max – fully automated for IHC.
AHSP / EDRF Antibody - Western Blot of rabbit anti-AHSP antibody. Lane 1: Recombinant hAHSP (2 ng). Lane 2: Recombinant mAHSP (2 ng). Lane 3: RBC Lysates Mouse #24 - GFP. Lane 4: RBC Lysates Mouse #25 - GFP. Lane 5: RBC Lysates Mouse #16 - hAHSP. Lane 6: RBC Lysates Mouse #17 - hAHSP. Lane 7: RBC Lysates Mouse #22 - hAHSP. Lane 8: RBC Lysates Mouse #19 - hAHSP. Lane 9: RBC Lysates Mouse #20 - hAHSP. Load: if not described differently, 10 ng per lane. Primary antibody: hAHSP antibody, Beta-Actin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: 5% dry milk 30 min at RT. Predicted/Observed size: ~12kDa. Other band(s): none.
AHSP / EDRF Antibody - Western Blot of rabbit anti-AHSP antibody. Lane 1: Recombinant hAHSP (10ng). Lane 2: Recombinant mAHSP (10ng). Lane 3: mice Spleen cells transfected with TNS9.2-hAHSP. Lane 4: mice Spleen cells transfected with TNS9.2 control vector. Lane 5: mice Spleen cells transfected with TNS9.2-hAHSP fractionated by MACS using Ter119+ microbeads. Lane 6: mice Spleen cells transfected with TNS9.2 control vector fractionated by Ter119+. Lane 7: mice Spleen cells transfected with TNS9.2-hAHSP fractionated by Ter119-. Lane 8: Spleen cells from mice transduced with TNS9.2 control vector fractionated by Ter119-. Load: 10 ng per lane. Primary antibody: AHSP antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: 5% dry milk 30 min at RT. Predicted/Observed size: ~12kDa. Other band(s): none.
AHSP / EDRF Antibody - Immunohistochemistry of rabbit anti-AHSP antibody. Tissue: A. Primary myelofibrosis, H&E. B. CD235a stains both nucleated EPs and mature, anucleate RBCs. AHSP C. and CD71 D. variably stain megakaryocytes and also stain nucleated EPs. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max – fully automated for IHC.
AHSP / EDRF Antibody - Immunohistochemistry of rabbit anti-AHSP antibody. Tissue: AHSP A. stains residual EPs and not lymphoma cells in DLBCL, whereas CD71 B. stains both lymphomacells and EPs. C. Corresponding H&E. AHSP D. does not metastatic carcinoma, whereas CD71 E. does. F. Corresponding H&E. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max – fully automated for IHC.
AHSP / EDRF Antibody - Immunohistochemistry of rabbit anti-AHSP antibody. Tissue: Giant pronormoblasts are evident in parvoviral infection (H&E, A). B. CD235a does not stain giant pronormoblasts. AHSP C. and CD71 D. stain giant pronormoblasts. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max – fully automated for IHC.
AHSP / EDRF Antibody - Immunohistochemistry of rabbit anti-AHSP antibody. Tissue: A. Normal bone marrow, H&E. B. CD235a stains both nucleated EPs and mature, anucleate RBCs. C. AHSP stains nucleated EPs, but not mature, anucleate RBCs. D. CD71 stains nucleated EPs, but not mature, anucleate RBCs. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max – fully automated for IHC.
AHSP / EDRF Antibody - Immunohistochemistry of rabbit anti-AHSP antibody. Tissue: A. Acute erythroleukemia, H&E. B. CD235a stains erythroid blasts and mature, anucleate RBCs. C. AHSP stains erythroid blasts. D. CD71 stains erythroid blasts. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max – fully automated for IHC.
AHSP / EDRF Antibody - Immunohistochemistry of rabbit anti-AHSP antibody. Tissue: AHSP stains residual EPs and not myeloid blasts in acute myeloid leukemia with minimal differentiation (A), whereas CD71 stains both myeloid blasts and EPs (B). AHSP does not stain myeloid blasts in acute myelomonocytic leukemia (D), whereas CD71 does (E). C and F are corresponding H&Es, respectively. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max – fully automated for IHC.
AHSP / EDRF Antibody - Western Blot of rabbit anti-AHSP antibody. Lane 1: Recombinant hAHSP (2 ng). Lane 2: Recombinant mAHSP (2 ng). Lane 3: RBC Lysates Mouse #24 - GFP. Lane 4: RBC Lysates Mouse #25 - GFP. Lane 5: RBC Lysates Mouse #16 - hAHSP. Lane 6: RBC Lysates Mouse #17 - hAHSP. Lane 7: RBC Lysates Mouse #22 - hAHSP. Lane 8: RBC Lysates Mouse #19 - hAHSP. Lane 9: RBC Lysates Mouse #20 - hAHSP. Load: if not described differently, 10 ng per lane. Primary antibody: hAHSP antibody, Beta-Actin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: 5% dry milk 30 min at RT. Predicted/Observed size: ~12kDa. Other band(s): none.
AHSP / EDRF Antibody - Western Blot of rabbit anti-AHSP antibody. Lane 1: Recombinant hAHSP (10ng). Lane 2: Recombinant mAHSP (10ng). Lane 3: mice Spleen cells transfected with TNS9.2-hAHSP. Lane 4: mice Spleen cells transfected with TNS9.2 control vector. Lane 5: mice Spleen cells transfected with TNS9.2-hAHSP fractionated by MACS using Ter119+ microbeads. Lane 6: mice Spleen cells transfected with TNS9.2 control vector fractionated by Ter119+. Lane 7: mice Spleen cells transfected with TNS9.2-hAHSP fractionated by Ter119-. Lane 8: Spleen cells from mice transduced with TNS9.2 control vector fractionated by Ter119-. Load: 10 ng per lane. Primary antibody: AHSP antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: 5% dry milk 30 min at RT. Predicted/Observed size: ~12kDa. Other band(s): none.
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Polyclonal Rabbit anti‑Human AHSP / EDRF Antibody (Liquid, IHC, WB) LS‑C744601

Polyclonal Rabbit anti‑Human AHSP / EDRF Antibody (Liquid, IHC, WB) LS‑C744601

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Antibody:
AHSP / EDRF Rabbit anti-Human Polyclonal (Liquid) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Liquid
Price
Catalog Number
$304
LS-C744601-25
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
AHSP / EDRF Rabbit anti-Human Polyclonal (Liquid) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Liquid

Specifications

Description
EDRF antibody LS-C744601 is an unconjugated rabbit polyclonal antibody to EDRF (AHSP) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB.
Target
Human AHSP / EDRF
Synonyms
AHSP | EDRF | Erythroid-associated factor | ERAF | Erythroid associated factor
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Liquid
Immunogen
Anti-AHSP Antibody was produced from whole rabbit serum prepared by repeated immunizations with the full length human AHSP protein.
Specificity
A BLAST analysis was used to suggest cross reactivity with human and mouse. Cross-reactivity with AHSP from other sources have not been determined.
Applications
  • IHC (1:8000)
  • Western blot (1:500 - 1:1000)
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AHSP / EDRF
Q9NZD4 NM_016633 NP_057717.1

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Requested From: United States
Date Requested: 4/24/2024
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