Human AGRP

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Biotin

Immunoaffinity purified

Unmodified

Sf 21-derived, recombinant human Agouti-Related Protein.

Recognizes human AgRP. ~40% cross-reactivity with rmAgRP.

• Western blot (0.1 - 0.2 µg/ml)
• ELISA

Suitable for use in Western Blot. Using plates coated with 100 ul/well of the capture antibody, in combination with 100 ul/well of the detection antibody at 50 ng/ml, an ELISA for sample volumes of 100 ul can be obtained. To arrive at the optimal dose range for. this ELISA, set up a two-fold dilution series of the protein standard stAgRPing with 4 ng/ml. Western Blot: 0.1-0.2 ug/ml. The detection limit for rhAgRP is approximately 1 ng/lane under non-reducing and reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 0.2 um sterile-filtered solution in PBS, 50 ug BSA

Sterile TBS, pH 7.3, 0.1% BSA.

Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O00253 NCBI: NM_001138 NP_001129.1