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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AGAP2 / PIKE Antibody (C‑Terminus) IHC‑plus™ LS‑B134 Best Seller
Note: This antibody replaces LS-C435, LS-C38227
PIKE antibody LS-B134 is an unconjugated rabbit polyclonal antibody to PIKE (AGAP2) from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B134-50
50 µg (1 mg/ml)
$425

Popular AGAP2 / PIKE Products

Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B338 concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
CENTG1 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The CENTG1 antibody detected the CENTG1 protein (arrow).
Species: Human, Mouse
Applications: Western blot
Anti-AGAP2 / PIKE antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4387 concentration 3.75 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

PIKE antibody LS-B134 is an unconjugated rabbit polyclonal antibody to PIKE (AGAP2) from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About AGAP2 / PIKE
Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. Q99490 NM_014770 NP_055585.1

AGAP2 Antibody, AGAP-2 Antibody, Centaurin, gamma 1 Antibody, Cnt-g1 Antibody, Centaurin-gamma-1 Antibody, KIAA0167 Antibody, GGAP2 Antibody, PIKE Antibody, CENTG1 Antibody


Specifications

Target
Human AGAP2 / PIKE
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30070 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B134. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Anti-PIKE will detect both PIKE-L and PIKE-A isoforms.
Usage
Immunohistochemistry: LS-B134 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B134 was determined to be 20 ug/ml.
Presentation
PBS, 0.02% Sodium Azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-AGAP2 / PIKE antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B134 concentration 20 ug/ml.
Anti-AGAP2 / PIKE antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B134 concentration 20 ug/ml.

Immunohistochemistry

Anti-AGAP2 / PIKE antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B134 concentration 20 ug/ml.
Anti-AGAP2 / PIKE antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B134 concentration 20 ug/ml.

Immunohistochemistry

Anti-AGAP2 / PIKE antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B134 concentration 20 ug/ml.
Anti-AGAP2 / PIKE antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B134 concentration 20 ug/ml.

Immunohistochemistry

Anti-AGAP2 / PIKE antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B134 concentration 20 ug/ml.
Anti-AGAP2 / PIKE antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B134 concentration 20 ug/ml.

Requested From: United States
Date Requested: 9/21/2018

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