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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AGAP2 / PIKE Antibody (aa20‑60) LS‑C148542
PIKE antibody LS-C148542 is an unconjugated rabbit polyclonal antibody to PIKE (AGAP2) from human and monkey. Validated for IHC and WB.
Catalog
Size
Price
LS-C148542-100
100 µg
$335

Popular AGAP2 / PIKE Products

Anti-AGAP2 / PIKE antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B134 concentration 20 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B338 concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
CENTG1 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The CENTG1 antibody detected the CENTG1 protein (arrow).
Species: Human, Mouse
Applications: Western blot
Anti-AGAP2 / PIKE antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4387 concentration 3.75 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

PIKE antibody LS-C148542 is an unconjugated rabbit polyclonal antibody to PIKE (AGAP2) from human and monkey. Validated for IHC and WB.
About AGAP2 / PIKE
Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. Q99490 NM_014770 NP_055585.1

AGAP2 Antibody, AGAP-2 Antibody, Centaurin, gamma 1 Antibody, Cnt-g1 Antibody, Centaurin-gamma-1 Antibody, KIAA0167 Antibody, GGAP2 Antibody, PIKE Antibody, CENTG1 Antibody


Specifications

Target
Human AGAP2 / PIKE
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (2 - 3 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
AGAP2 / PIKE antibody was raised against a portion of amino acids 20-60 of human AGAP2.
Epitope
aa20-60
Specificity
Human AGAP2 / PIKE.
Presentation
PBS, 0.05% Sodium Azide, 0.05% BSA
Storage
Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.
Restrictions
For research use only.

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Images

Immunohistochemistry

IHC ofAGAP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and LS-C148542 (bottom left, right) at 5 ug/ml.
IHC ofAGAP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and LS-C148542 (bottom left, right) at 5 ug/ml.

Western blot

Western blot of AGAP2 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using LS-C148542 at 2.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.
Western blot of AGAP2 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using LS-C148542 at 2.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.

Immunohistochemistry

IHC ofAGAP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and LS-C148542 (bottom left, right) at 5 ug/ml.
IHC ofAGAP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and LS-C148542 (bottom left, right) at 5 ug/ml.

Western blot

Western blot of AGAP2 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using LS-C148542 at 2.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.
Western blot of AGAP2 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using LS-C148542 at 2.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.

Immunohistochemistry

IHC ofAGAP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and LS-C148542 (bottom left, right) at 5 ug/ml.
IHC ofAGAP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and LS-C148542 (bottom left, right) at 5 ug/ml.

Western blot

Western blot of AGAP2 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using LS-C148542 at 2.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.
Western blot of AGAP2 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using LS-C148542 at 2.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.

Immunohistochemistry

IHC ofAGAP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and LS-C148542 (bottom left, right) at 5 ug/ml.
IHC ofAGAP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and LS-C148542 (bottom left, right) at 5 ug/ml.

Western blot

Western blot of AGAP2 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using LS-C148542 at 2.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.
Western blot of AGAP2 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using LS-C148542 at 2.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.

Requested From: United States
Date Requested: 9/23/2018

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