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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AES / Groucho Antibody (N‑Terminus) LS‑C169505
Groucho antibody LS-C169505 is an unconjugated rabbit polyclonal antibody to Groucho (AES) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C169505-50
50 µg
$365

Popular AES / Groucho Products

Anti-AES antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Anti-AES antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Anti-Groucho antibody IHC of human colon, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Species: Human, Mouse, Rat, Bovine, Dog, Hamster, Xenopus
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunofluorescence analysis of A549 cells.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot
Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA

Product Description

Groucho antibody LS-C169505 is an unconjugated rabbit polyclonal antibody to Groucho (AES) from human, mouse, rat and other species. Validated for WB.

Specifications

Target
Mouse AES / Groucho
Synonyms
AES, AES-1, AES-2, Amino enhancer of split, ESP1, Groucho, GRG, Protein ESP1, TLE5, Protein GRG, gp130-associated protein GAM, GRG5
Host
Rabbit
Reactivity
Mouse, Human, Monkey, Rat, Bovine, Dog, Guinea pig, Xenopus (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
AES / Groucho antibody was raised against synthetic peptide from N-Terminus of mouse Aes (P63002, NP_034477). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Opossum, Guinea pig, Platypus, Xenopus, Frog (100%).
Blocking Peptide
LS-E11303 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C169505. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Mouse Groucho
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About AES / Groucho
Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development. Q08117 NM_198969 NP_945320.1


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Images

Western blot

 This image was taken for the unconjugated form of this product. Other forms have not been tested.
This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

 This image was taken for the unconjugated form of this product. Other forms have not been tested.
This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

 This image was taken for the unconjugated form of this product. Other forms have not been tested.
This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

 This image was taken for the unconjugated form of this product. Other forms have not been tested.
This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/12/2018

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