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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AES / Groucho Antibody IHC‑plus™ LS‑B1326
Note: This antibody replaces LS-C19535, LS-C126636
Groucho antibody LS-B1326 is an unconjugated rabbit polyclonal antibody to Groucho (AES) from human and mouse. Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1326-50
50 µg (1 mg/ml)
$425
Description
Groucho antibody LS-B1326 is an unconjugated rabbit polyclonal antibody to Groucho (AES) from human and mouse. Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human AES / Groucho
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (2 - 4 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
AES / Groucho antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human AES. The immunogen is located within the first 50 amino acids of AES.
Blocking Peptide
LS-E30176 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B1326. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
AES antibody can be used for the detection of AES by Western blot at 2 - 4 ug/mL. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
Presentation
PBS containing 0.02% thimerosal.
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About AES / Groucho
Q08117 NM_198969 NP_945320.1

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Images

Immunohistochemistry

Anti-AES antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AES antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-AES antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AES antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of AES in 293 cells with AES antibody at 10 ug/ml.
Immunocytochemistry of AES in 293 cells with AES antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of AES in 293 cells with AES antibody at 20 ug/ml.
Immunofluorescence of AES in 293 cells with AES antibody at 20 ug/ml.

Western blot

Western blot of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 ug/ml.
Western blot of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 ug/ml.

Immunohistochemistry

Anti-AES antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AES antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-AES antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AES antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of AES in 293 cells with AES antibody at 10 ug/ml.
Immunocytochemistry of AES in 293 cells with AES antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of AES in 293 cells with AES antibody at 20 ug/ml.
Immunofluorescence of AES in 293 cells with AES antibody at 20 ug/ml.

Western blot

Western blot of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 ug/ml.
Western blot of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 ug/ml.

Immunohistochemistry

Anti-AES antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AES antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-AES antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AES antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of AES in 293 cells with AES antibody at 10 ug/ml.
Immunocytochemistry of AES in 293 cells with AES antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of AES in 293 cells with AES antibody at 20 ug/ml.
Immunofluorescence of AES in 293 cells with AES antibody at 20 ug/ml.

Western blot

Western blot of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 ug/ml.
Western blot of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 ug/ml.

Immunohistochemistry

Anti-AES antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AES antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-AES antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AES antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of AES in 293 cells with AES antibody at 10 ug/ml.
Immunocytochemistry of AES in 293 cells with AES antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of AES in 293 cells with AES antibody at 20 ug/ml.
Immunofluorescence of AES in 293 cells with AES antibody at 20 ug/ml.

Western blot

Western blot of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 ug/ml.
Western blot of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 ug/ml.

Requested From: United States
Date Requested: 1/16/2019
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