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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AES / Groucho Antibody (C‑Terminus) IHC‑plus™ LS‑B441 Best Seller
Note: This antibody replaces LS-C19534, LS-C56769, LS-C56868, LS-C34910, LS-C42326
Groucho antibody LS-B441 is an unconjugated rabbit polyclonal antibody to Groucho (AES) from human, mouse and rat. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B441-50
50 µg (1 mg/ml)
$425
Description
Groucho antibody LS-B441 is an unconjugated rabbit polyclonal antibody to Groucho (AES) from human, mouse and rat. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human AES / Groucho
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30175 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B441. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Usage
Immunohistochemistry: LS-B441 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B441 was determined to be 2.5 ug/ml.
Presentation
PBS, 0.02% Sodium Azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
About AES / Groucho
Q08117 NM_198969 NP_945320.1

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Images

Immunohistochemistry

Anti-AES antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AES antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of AES in HeLa cells with AES antibody at 10 ug/ml.
Immunocytochemistry of AES in HeLa cells with AES antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of AES in HeLa cells with AES antibody at 20 ug/ml.
Immunofluorescence of AES in HeLa cells with AES antibody at 20 ug/ml.

Western blot

Western blot of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 ug/ml.
Western blot of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 ug/ml.

Immunohistochemistry

Anti-AES antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AES antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of AES in HeLa cells with AES antibody at 10 ug/ml.
Immunocytochemistry of AES in HeLa cells with AES antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of AES in HeLa cells with AES antibody at 20 ug/ml.
Immunofluorescence of AES in HeLa cells with AES antibody at 20 ug/ml.

Western blot

Western blot of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 ug/ml.
Western blot of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 ug/ml.

Immunohistochemistry

Anti-AES antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AES antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of AES in HeLa cells with AES antibody at 10 ug/ml.
Immunocytochemistry of AES in HeLa cells with AES antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of AES in HeLa cells with AES antibody at 20 ug/ml.
Immunofluorescence of AES in HeLa cells with AES antibody at 20 ug/ml.

Western blot

Western blot of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 ug/ml.
Western blot of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 ug/ml.

Immunohistochemistry

Anti-AES antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AES antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of AES in HeLa cells with AES antibody at 10 ug/ml.
Immunocytochemistry of AES in HeLa cells with AES antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of AES in HeLa cells with AES antibody at 20 ug/ml.
Immunofluorescence of AES in HeLa cells with AES antibody at 20 ug/ml.

Western blot

Western blot of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 ug/ml.
Western blot of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 ug/ml.

Requested From: United States
Date Requested: 11/19/2018
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