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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AES / Groucho Antibody (C‑Terminus, Biotin) LS‑C434223
Groucho antibody LS-C434223 is a biotin-conjugated rabbit polyclonal antibody to Groucho (AES) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C434223-100
100 µl (1 mg/ml)
$335

Popular AES / Groucho Products

Anti-AES antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Anti-AES antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Anti-Groucho antibody IHC of human colon, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Species: Human, Mouse, Rat, Bovine, Dog, Hamster, Xenopus
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunofluorescence analysis of A549 cells.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot
Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA

Product Description

Groucho antibody LS-C434223 is a biotin-conjugated rabbit polyclonal antibody to Groucho (AES) from human, mouse, rat and other species. Validated for WB.

Specifications

Target
Human AES / Groucho
Synonyms
AES, AES-1, AES-2, Amino enhancer of split, ESP1, Groucho, GRG, Protein ESP1, TLE5, Protein GRG, gp130-associated protein GAM, GRG5
Host
Rabbit
Reactivity
Human, Chimpanzee, Monkey, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with FITC, HRP.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
AES / Groucho antibody was raised against synthetic peptide from C-Terminus of human AES (Q08117, NP_001121). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Mouse, Rat, Dog, Bovine (100%).
Epitope
C-Terminus
Specificity
Human AES / GRG / Groucho
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
About AES / Groucho
Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development. Q08117 NM_198969 NP_945320.1


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Images

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/14/2018

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