Groucho antibody LS-C109651 is an unconjugated rabbit polyclonal antibody to Groucho (AES) from human, mouse, rat and other species. Validated for WB.
Human AES / Groucho
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Chicken, Xenopus, Zebrafish
(tested or 100% immunogen sequence identity)
Also available conjugated with HRP
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
AES / Groucho antibody was raised against synthetic peptide located between aa143-192 of human AES (Q14CJ1, NP_945320). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Opossum, Guinea pig, Chicken, Xenopus, Frog (100%); Zebrafish (85%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109651. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human AES / GRG / Groucho
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
For research use only.