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Catalog Number Size Price
LS-C20327-50 50 µl $445 
Antibody

ADRA2A Antibody LS‑C20327

ADRA2A Antibody LS‑C20327

Antibody:
Rabbit Polyclonal to Rat ADRA2A
Application:
IHC, ICC, IF
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$445 
LS-C20327-50
50 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Rat ADRA2A
Application:
IHC, ICC, IF
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ADRA2A antibody LS-C20327 is an unconjugated rabbit polyclonal antibody to rat ADRA2A. Validated for ICC, IF and IHC.
Target
Rat ADRA2A
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:100 - 1:500)
  • ICC
  • Immunofluorescence
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
(C)KASRWRGRGNREKR; Cysteine added for conjugation. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Elephant, Panda, Cat, Bovine, Pig, Opossum, Guinea pig, Lizard (93%); Pufferfish, Zebrafish (86%).
Specificity
Recognizes rat alpha 2a Adrenergic Receptor. Shows positive staining in rat spinal cord dorsal horn, ventral horn motor neurons and retina. When examined in other brain regions known to express alpha 2a, staining was negative. Species cross-reactivity: Mouse and human.
Usage
Suitable for use in Immunohistochemistry and Immunocytochemistry. Immunohistochemistry: 1:100-1:500; Antiserum was used on perfusion fixed tissue. Perfusion: 1) Calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4?C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and cover-slipped. Staining was examined using fluorescence microscopy.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADRA2A
P08913 NM_000681 NP_000672.3

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Requested From: United States
Date Requested: 12/14/2019