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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ADGRE5 / CD97 Antibody (N‑Terminus) IHC‑plus™ LS‑A2644
CD97 antibody LS-A2644 is an unconjugated rabbit polyclonal antibody to human CD97 (ADGRE5). Validated for ICC and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A2644-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular ADGRE5 / CD97 Products

Anti-CD97 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1780 concentration 10 ug/ml.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot
Anti-CD97 Antibody - Western Blot. Western blot of Protein A purified anti-CD97 antibody shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. The left lane contains lysate from cells transfected with control DNA. The right lane contains lysate from COS cells expressing Fc-CD97- (5EGF). No staining was noted from bone marrow lysates taken from CD97 knockout mice. The identity of the band at ~65 kD appearing in all lanes is not known. The formation of the CD97 complex is currently under investigation. Approximately 10 ul of lysate was used in each lane. A 1:1000 dilution of the primary antibody was used. The image was processed using a 10-sec exposure. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.
Species: Mouse, Human
Applications: Immunofluorescence, Western blot
Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, ELISA
Antibody
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA

Product Description

CD97 antibody LS-A2644 is an unconjugated rabbit polyclonal antibody to human CD97 (ADGRE5). Validated for ICC and IHC. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human ADGRE5 / CD97
Synonyms
ADGRE5, CD97, CD97 molecule, Leukocyte antigen CD97, TM7LN1, CD97 antigen
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ADGRE5 / CD97 antibody was raised against synthetic 19 amino acid peptide from N-terminal extracellular domain of human CD97. Percent identity with other species by BLAST analysis: Human (100%); Monkey (95%).
Blocking Peptide
LS-E28584 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A2644. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human CD97. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MAP3K12 (58%).
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About ADGRE5 / CD97
ADGRE5 / CD97 is a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. P48960 NM_078481 NP_510966.1


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Images

Immunohistochemistry

Anti-CD97 antibody LS-A2644 IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody LS-A2644 IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CD97 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CD97 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CD97 antibody IHC of human Thyroid, Papillary Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Thyroid, Papillary Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunocytochemistry

Anti-CD97 antibody LS-A2644 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.
Anti-CD97 antibody LS-A2644 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.

Immunohistochemistry

Anti-CD97 antibody LS-A2644 IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody LS-A2644 IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CD97 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CD97 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CD97 antibody IHC of human Thyroid, Papillary Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Thyroid, Papillary Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunocytochemistry

Anti-CD97 antibody LS-A2644 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.
Anti-CD97 antibody LS-A2644 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.

Immunohistochemistry

Anti-CD97 antibody LS-A2644 IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody LS-A2644 IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CD97 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CD97 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CD97 antibody IHC of human Thyroid, Papillary Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Thyroid, Papillary Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunocytochemistry

Anti-CD97 antibody LS-A2644 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.
Anti-CD97 antibody LS-A2644 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.

Immunohistochemistry

Anti-CD97 antibody LS-A2644 IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody LS-A2644 IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CD97 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CD97 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CD97 antibody IHC of human Thyroid, Papillary Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Thyroid, Papillary Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunocytochemistry

Anti-CD97 antibody LS-A2644 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.
Anti-CD97 antibody LS-A2644 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.

Requested From: United States
Date Requested: 10/17/2018

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