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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ADGRE2 / EMR2 Antibody (clone 2A1, RPE) LS‑C188292
EMR2 antibody LS-C188292 is an RPE-conjugated mouse monoclonal antibody to human EMR2 (ADGRE2). Validated for Flow, IHC, IP and WB.
Catalog
Size
Price
LS-C188292-25
25 tst
$235
LS-C188292-100
100 tst
$435
Description
EMR2 antibody LS-C188292 is an RPE-conjugated mouse monoclonal antibody to human EMR2 (ADGRE2). Validated for Flow, IHC, IP and WB.
Target
Human ADGRE2 / EMR2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [2A1]
Conjugations
R. Phycoerythrin. Also available Unconjugated.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:1)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ADGRE2 / EMR2 antibody was raised against nIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA.
Specificity
Recognizes human EMR2, a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2. Clone 2A1 specifically recognizes the stalk region of EMR2.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.09% sodium azide, 1% BSA, 5% sucrose
Reconstitution
25 tst or 100 tst Distilled water
Storage
Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.
Restrictions
For research use only.
About ADGRE2 / EMR2
Q9UHX3 NM_013447 NP_038475.2

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Images

Flow Cytometry

Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312: RPE This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312: RPE This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312: RPE This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312: RPE This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312: RPE This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312: RPE This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312: RPE This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312: RPE This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human CD312. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/15/2018
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