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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ADGRA2 / GPR124 Antibody (N‑Terminus) IHC‑plus™ LS‑A2167
GPR124 antibody LS-A2167 is an unconjugated rabbit polyclonal antibody to GPR124 (ADGRA2) from human, bat, dog and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A2167-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular ADGRA2 / GPR124 Products

Anti-GPR124 antibody LS-A2171 IHC of human colon carcinoma, neoplastic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin
Anti-GPR124 / TEM5 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin
Immunofluorescence analysis of HeLa cells, using GPR124 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human, Mouse
Applications: Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry image of paraffin-embedded human liver cancer at a dilution of 1:100
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA

Product Description

GPR124 antibody LS-A2167 is an unconjugated rabbit polyclonal antibody to GPR124 (ADGRA2) from human, bat, dog and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human ADGRA2 / GPR124
Synonyms
ADGRA2, G protein-coupled receptor 124, G-protein coupled receptor 124, TEM5, GPR124, KIAA1531, Tumor endothelial marker 5
Host
Rabbit
Reactivity
Human, Monkey, Bat, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Hamster, Chicken, Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (4 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ADGRA2 / GPR124 antibody was raised against synthetic 17 amino acid peptide from N-terminal extracellular domain of human GPR124. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Dog, Bat, Elephant, Panda, Horse, Rabbit, Pig, Opossum (100%); Mouse, Rat, Hamster, Turkey, Chicken, Xenopus (94%); Monkey, Bovine, Platypus, Lizard (82%).
Blocking Peptide
LS-E28517 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A2167. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human GPR124. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GPR125 (82%).
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About ADGRA2 / GPR124
Tumor endothelial marker 5 is an Orphan-U GPCR with an unknown ligand. Tumor Endothelial Marker 5, like GPR125, has a leucine rich repeat (LRR), an immunoglobulin (Ig) domain, and a hormone-binding domain (HBD). The Ig domain shows similarities to motilin and titin, while the LRR domain shows similarities to LRIG1 and SLIT1-2. Q96PE1 AY181242 NP_116166.9


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Images

Immunohistochemistry

Anti-GPR124 / TEM5 antibody IHC of human Prostate, Carcinoma Endothelial Cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 / TEM5 antibody IHC of human Prostate, Carcinoma Endothelial Cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR124 antibody LS-A2167 IHC of human colon carcinoma, neoplastic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 antibody LS-A2167 IHC of human colon carcinoma, neoplastic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR124 / TEM5 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 / TEM5 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR124 / TEM5 antibody IHC of human Skin, Kaposis Sarcoma capillary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 / TEM5 antibody IHC of human Skin, Kaposis Sarcoma capillary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR124 / TEM5 antibody IHC of human Prostate, Carcinoma Endothelial Cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 / TEM5 antibody IHC of human Prostate, Carcinoma Endothelial Cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR124 antibody LS-A2167 IHC of human colon carcinoma, neoplastic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 antibody LS-A2167 IHC of human colon carcinoma, neoplastic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR124 / TEM5 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 / TEM5 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR124 / TEM5 antibody IHC of human Skin, Kaposis Sarcoma capillary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 / TEM5 antibody IHC of human Skin, Kaposis Sarcoma capillary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR124 / TEM5 antibody IHC of human Prostate, Carcinoma Endothelial Cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 / TEM5 antibody IHC of human Prostate, Carcinoma Endothelial Cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR124 antibody LS-A2167 IHC of human colon carcinoma, neoplastic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 antibody LS-A2167 IHC of human colon carcinoma, neoplastic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR124 / TEM5 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 / TEM5 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR124 / TEM5 antibody IHC of human Skin, Kaposis Sarcoma capillary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 / TEM5 antibody IHC of human Skin, Kaposis Sarcoma capillary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR124 / TEM5 antibody IHC of human Prostate, Carcinoma Endothelial Cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 / TEM5 antibody IHC of human Prostate, Carcinoma Endothelial Cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR124 antibody LS-A2167 IHC of human colon carcinoma, neoplastic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 antibody LS-A2167 IHC of human colon carcinoma, neoplastic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR124 / TEM5 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 / TEM5 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR124 / TEM5 antibody IHC of human Skin, Kaposis Sarcoma capillary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR124 / TEM5 antibody IHC of human Skin, Kaposis Sarcoma capillary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 10/16/2018

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