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Catalog Number Size Price
LS-C204505-100 100 µl (1 mg/ml) $417 
ADCY3 / Adenylate Cyclase 3 Antibody - Confocal image of mixed rat neuron-glial cultures stained with our rabbit polyclonal antibody to ACIII (red) and our mouse monoclonal antibody to aII-spectrin (MCA-3D7 green). Note the strong and clean staining of neuronal cilia. Since aII-spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody. The aII-spectrin antibody is also an excellent marker of neuronal plasma membranes.
ADCY3 / Adenylate Cyclase 3 Antibody - Western blots of rat olfactory epithelium (OE) and frontal cortex (FC). The antibody stains bands at about 200kDa in olfactory epithelium which is rich in cilia. Fewer cilia are found in frontal cortex, and the protein is less heavily glycosylated, and a less prominent band is seen at about 160kDa. Data generated in the laboratory of Matt Sarkisian in the University of Florida.
ADCY3 / Adenylate Cyclase 3 Antibody - Confocal image of mixed rat neuron-glial cultures stained with our rabbit polyclonal antibody to ACIII (red) and our mouse monoclonal antibody to aII-spectrin (MCA-3D7 green). Note the strong and clean staining of neuronal cilia. Since aII-spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody. The aII-spectrin antibody is also an excellent marker of neuronal plasma membranes.
ADCY3 / Adenylate Cyclase 3 Antibody - Western blots of rat olfactory epithelium (OE) and frontal cortex (FC). The antibody stains bands at about 200kDa in olfactory epithelium which is rich in cilia. Fewer cilia are found in frontal cortex, and the protein is less heavily glycosylated, and a less prominent band is seen at about 160kDa. Data generated in the laboratory of Matt Sarkisian in the University of Florida.
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Polyclonal Rabbit anti‑Rat ADCY3 / Adenylate Cyclase 3 Antibody (aa1125‑1144, IF, WB) LS‑C204505

Polyclonal Rabbit anti‑Rat ADCY3 / Adenylate Cyclase 3 Antibody (aa1125‑1144, IF, WB) LS‑C204505

Note: This antibody replaces LS-C180198
Antibody:
ADCY3 / Adenylate Cyclase 3 Rabbit anti-Rat Polyclonal (aa1125-1144) Antibody
Application:
ICC, IF, WB
Reactivity:
Rat, Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$417
LS-C204505-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ADCY3 / Adenylate Cyclase 3 Rabbit anti-Rat Polyclonal (aa1125-1144) Antibody
Application:
ICC, IF, WB
Reactivity:
Rat, Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Adenylate Cyclase 3 antibody LS-C204505 is an unconjugated rabbit polyclonal antibody to Adenylate Cyclase 3 (ADCY3) (aa1125-1144) from rat. It is reactive with human, mouse and rat. Validated for ICC, IF and WB. Cited in 2 publications.
Target
Rat ADCY3 / Adenylate Cyclase 3
Synonyms
ADCY3 | AC-III | AcIII | Adenylyl cyclase 3 | AC3 | Adenylate cyclase type III | ATP pyrophosphate-lyase 3 | Adenylate cyclase 3 | Adenylate cyclase type 3 | Adenylyl cyclase, type III | KIAA0511
Host
Rabbit
Reactivity
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide PAAFPNGSSVTLPHQVVDNP with a cysteine residue added to the N-Terminus to allow coupling to KLH. The peptide corresponds to the extreme C-Terminus of rat molecule. Percent identity by BLAST analysis: Mouse, Rat (100%); Horse, Rabbit (95%); Pig (85%); Human, Chimpanzee, Guinea pig (80%).
Epitope
aa1125-1144
Specificity
Mouse and rat Adenylate Cyclase 3.
Applications
  • ICC (1:500 - 1:1000)
  • Immunofluorescence (1:500 - 1:1000)
  • Western blot (1:5000 - 1:10000)
Usage
The antibody can be diluted to 1:500-1:1000 for immunofluorescence staining and 1:5000-1:10000 for western blotting. On western blots look for a major bands at 160-1:200kDa, depending on the tissue.
Presentation
PBS, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADCY3 / Adenylate Cyclase 3
O60266 NM_004036 NP_004027.2

LSBio Ratings

ADCY3 / Adenylate Cyclase 3 Antibody (aa1125-1144) for ICC, IF/Immunofluorescence, WB/Western LS-C204505 has an LSBio Rating of
Publications (4.1)

Publications (2)

Ankmy2 Prevents Smoothened-Independent Hyperactivation of the Hedgehog Pathway via Cilia-Regulated Adenylyl Cyclase Signaling. Bandarigoda Nipunika Somatilaka, Sun-Hee Hwang, Vivek Reddy Palicharla, Kevin Andrew White, Hemant Badgandi, John Michael Shelton, Saikat Mukhopadhyay. Developmental cell. 2020 September;54:710-726e8.

Phylogenetic profiling and cellular analyses of ARL16 reveal roles in traffic of IFT140 and INPP5E. Skylar I Dewees, Romana Vargová, Katherine R Hardin, Rachel E Turn, Saroja Devi, Joshua Linnert, Uwe Wolfrum, Tamara Caspary, Marek Eliáš, Richard A Kahn. Molecular biology of the cell. 2022 April;33:ar33.

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Requested From: United States
Date Requested: 4/19/2024