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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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ADAM10 Antibody LS‑C289

Catalog Number Size Price
LS-C289-0.1 0.1 mg (1 mg/ml) $365 
Immunocytochemical staining of K562 cells using ADAM10 antibody at 2 µg/ml.
Immunofluorescence of ADAM10 in K562 cells with ADAM10 antibody at 10 ug/ml.
Western blot of ADAM10 in Jurkat whole cell lysate in the absence (1) or presence (2) of blocking peptide (Catalog no. 2051P) with ADAM10 antibody at 1 mg/ml.
Immunocytochemical staining of K562 cells using ADAM10 antibody at 2 µg/ml.
Immunofluorescence of ADAM10 in K562 cells with ADAM10 antibody at 10 ug/ml.
Western blot of ADAM10 in Jurkat whole cell lysate in the absence (1) or presence (2) of blocking peptide (Catalog no. 2051P) with ADAM10 antibody at 1 mg/ml.
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ADAM10 Antibody LS‑C289

Antibody:
Rabbit Polyclonal to Human ADAM10
Application:
IHC, ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$365 
LS-C289-0.1
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human ADAM10
Application:
IHC, ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ADAM10 antibody LS-C289 is an unconjugated rabbit polyclonal antibody to human ADAM10. Validated for ELISA, ICC, IF, IHC and WB.
Target
Human ADAM10
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC (2 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
a 17 amino acid peptide near the C-terminus of human ADAM10. The immunogen is located within the last 50 amino acids of ADAM10.
Blocking Peptide
LS-E29873 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-C289. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
ADAM10 can be used for detection of ADAM10 by Western blot at 1 - 2 ug/mL. This polyclonal antibody can also detect ADAM10 by immunohistochemistry at 2 ug/mL. For immunofluorescence start at 10 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: 85 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADAM10
O14672 NM_001110 NP_001101.1

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Western blot analysis of extracts of various cell lines, using ADAM10 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
Species: Human, Mouse, Rat
Applications: Western blot
Reactivity: Mouse
Range: 9.38-600 pg/ml

Requested From: United States
Date Requested: 8/24/2019
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