Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C745297-25 25 µl (1 mg/ml) $275 
ACTB / Beta Actin Antibody - Immunohistochemistry of rabbit Anti-Beta Actin Antibody. Tissue: sections 4.2 mm thick of rana pipiens tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Beta Actin antibody at 1:200 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Beta Actin is at the neuromuscular junction. Staining: Beta Actin as precipitated green signal with red and blue counterstain.
ACTB / Beta Actin Antibody - Western Blot of rabbit Anti-Beta Actin Antibody. Lane 1: molecular weight. Lane 2: human embryonic kidney 293. Lane 3: human lung carcinoma A549. Lane 4: mouse brain. Load: 35 µg per lane. Primary antibody: Beta Actin antibody at 1:1,500 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~42 kDa corresponding to beta Actin (arrowhead). Other band(s): none.
ACTB / Beta Actin Antibody - Immunohistochemistry of rabbit Anti-Beta Actin Antibody. Tissue: sections 4.2 mm thick of rana pipiens tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Beta Actin antibody at 1:200 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Beta Actin is at the neuromuscular junction. Staining: Beta Actin as precipitated green signal with red and blue counterstain.
ACTB / Beta Actin Antibody - Western Blot of rabbit Anti-Beta Actin Antibody. Lane 1: molecular weight. Lane 2: human embryonic kidney 293. Lane 3: human lung carcinoma A549. Lane 4: mouse brain. Load: 35 µg per lane. Primary antibody: Beta Actin antibody at 1:1,500 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~42 kDa corresponding to beta Actin (arrowhead). Other band(s): none.
1 of 2
2 of 2

Polyclonal Rabbit anti‑Human ACTB / Beta Actin Antibody (aa350‑375, IHC, IF, WB) LS‑C745297

Polyclonal Rabbit anti‑Human ACTB / Beta Actin Antibody (aa350‑375, IHC, IF, WB) LS‑C745297

Antibody:
ACTB / Beta Actin Rabbit anti-Human Polyclonal (aa350-375) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Goat, Guinea pig, Hamster, Pig, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745297-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
ACTB / Beta Actin Rabbit anti-Human Polyclonal (aa350-375) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Goat, Guinea pig, Hamster, Pig, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
Beta Actin antibody LS-C745297 is an unconjugated rabbit polyclonal antibody to Beta Actin (ACTB) (aa350-375) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB.
Target
Human ACTB / Beta Actin
Synonyms
ACTB | Actin, cytoplasmic 1 | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Goat, Guinea pig, Hamster, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin.
Epitope
aa350-375
Specificity
Reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
Applications
  • IHC
  • Immunofluorescence (1:500 - 1:2000)
  • Western blot (1:1000 - 1:4000)
  • ELISA (1:10000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTB / Beta Actin
P60709 NM_001101 NP_001092.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021