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Antibodies > Acrolein > LS-C775549
 
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Catalog Number Size Price
LS-C775549-100 100 µg (1 mg/ml) $497 
Acrolein Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Acrolein Antibody - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
Acrolein Antibody - Western Blot analysis of Acrolein-BSA Conjugate showing detection of 67 kDa Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Lane 1: Molecular Weight Ladder (MW). Lane 2: AcroleinBSA (0.5 µg). Lane 3: AcroleinBSA (2.0 µg). Lane 4: BSA (0.5 µg). Lane 5: BSA (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Acrolein Antibody - Mouse Anti-Acrolein Antibody [10A10] used in Western Blot (WB) on Cervical cancer cell line (HeLa) lysate
Acrolein Antibody - Flow Cytometry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
Acrolein Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Acrolein Antibody - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
Acrolein Antibody - Western Blot analysis of Acrolein-BSA Conjugate showing detection of 67 kDa Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Lane 1: Molecular Weight Ladder (MW). Lane 2: AcroleinBSA (0.5 µg). Lane 3: AcroleinBSA (2.0 µg). Lane 4: BSA (0.5 µg). Lane 5: BSA (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Acrolein Antibody - Mouse Anti-Acrolein Antibody [10A10] used in Western Blot (WB) on Cervical cancer cell line (HeLa) lysate
Acrolein Antibody - Flow Cytometry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
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Monoclonal Mouse anti‑All species Acrolein Antibody (clone 10A10, IF, WB) LS‑C775549

Monoclonal Mouse anti‑All species Acrolein Antibody (clone 10A10, IF, WB) LS‑C775549

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Antibody:
Acrolein Mouse Monoclonal (10A10) Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
All species
Format:
Unconjugated, Unmodified
Price
Catalog Number
$497
LS-C775549-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Acrolein Mouse Monoclonal (10A10) Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
All species
Format:
Unconjugated, Unmodified

Specifications

Description
Acrolein antibody LS-C775549 is an unconjugated mouse monoclonal antibody to all species Acrolein. Validated for ELISA, Flow, IF and WB.
Target
All species Acrolein
Host
Mouse
Reactivity
All species
Clonality
IgG1 Monoclonal
Clone
10A10
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).
Specificity
Specific for Acrolein modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Applications
  • Immunofluorescence (1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:50)
  • ELISA (1:1000)
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

Acrolein Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Acrolein Antibody (clone 10A10, Biotin) LS‑C775547
Species: All species
Applications: Immunofluorescence, Western blot, Flow Cytometry, ELISA
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Reactivity: Human
Range: 15.6-1000 pg/ml
AKAP11 / KIAA0629 Antibody - Western Blot; Sample: Recombinant AKAP11, Human.
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Complement C5a ELISA Kit
Monkey Complement C5a (Sandwich ELISA) ELISA Kit - LS-F34380
Reactivity: Monkey
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Reactivity: Human
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See all Acrolein Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
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