CD143 antibody LS-C3706 is an unconjugated mouse monoclonal antibody to CD143 (ACE) from human, rat, bovine and other species. Validated for ELISA, IHC and WB.
Human, Monkey, Rat, Bovine, Cat, Dog, Guinea pig, Rabbit
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- IHC - Paraffin
- Western blot (1:50 - 1:100)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
ACE / CD143 antibody was raised against denatured human kidney CD143 (Angiotensin converting enzyme).
Recognizes human CD143, also known as angiotensin-converting enzyme (ACE). CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of arterioles and lung capillaries), epithelial cells (especially in proximal renal tubules and in the small intestine), by some neuronal cells and variably on some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. Study of CD143 may be relevant to fertility (especially sperm-egg interactions), atherosclerosis and in drug targeting to the lung. This clone has been reported to recognize ACE in Western Blot. The antibody recognizes denatured ACE, binding to an epitope in the C domain. Species cross-reactivity: Human, monkey, rabbit, bovine, canine, feline, guinea pig, rat.
Suitable for use in Immunohistochemistry, Western Blot and. ELISA. Immunohistochemistry (Paraffin): 1:10-1:100. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended. Western Blot: 1:50-1:100. Positive control: Human Kidney.
Tissue Culture Supernatant, 0.1% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.