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Antibodies > ABI1 / SSH3BP1 > LS-C312249
 
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Catalog Number Size Price
LS-C312249-10 10 µg $318 
LS-C312249-100 100 µg (0.5 mg/ml) $470 
ABI1 / SSH3BP1 Antibody - IHC analysis of ABI using anti-ABI antibody. ABI was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ABI Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ABI1 / SSH3BP1 Antibody - IHC analysis of ABI using anti-ABI antibody. ABI was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ABI Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ABI1 / SSH3BP1 Antibody - ABI1 / ABI-1 antibody. IHC(P): Rat Brain Tissue.
ABI1 / SSH3BP1 Antibody - Western blot analysis of ABI-1 using anti-ABI-1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human THP-1 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABI-1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABI-1 at approximately 65KD. The expected band size for ABI-1 is at 55KD.
ABI1 / SSH3BP1 Antibody - WB of ABI1 / ABI-1 antibody.
ABI1 / SSH3BP1 Antibody - IHC analysis of ABI using anti-ABI antibody. ABI was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ABI Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ABI1 / SSH3BP1 Antibody - IHC analysis of ABI using anti-ABI antibody. ABI was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ABI Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ABI1 / SSH3BP1 Antibody - ABI1 / ABI-1 antibody. IHC(P): Rat Brain Tissue.
ABI1 / SSH3BP1 Antibody - Western blot analysis of ABI-1 using anti-ABI-1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human THP-1 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABI-1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABI-1 at approximately 65KD. The expected band size for ABI-1 is at 55KD.
ABI1 / SSH3BP1 Antibody - WB of ABI1 / ABI-1 antibody.
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Polyclonal Rabbit anti‑Human ABI1 / SSH3BP1 Antibody (aa483‑494, IHC, WB) LS‑C312249

Polyclonal Rabbit anti‑Human ABI1 / SSH3BP1 Antibody (aa483‑494, IHC, WB) LS‑C312249

Submit Review LSBio Guarantee
Note: This antibody replaces LS-C389235
Antibody:
ABI1 / SSH3BP1 Rabbit anti-Human Polyclonal (aa483-494) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Guinea pig, Horse, Pig, Rabbit, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312249-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ABI1 / SSH3BP1 Rabbit anti-Human Polyclonal (aa483-494) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Guinea pig, Horse, Pig, Rabbit, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
SSH3BP1 antibody LS-C312249 is an unconjugated rabbit polyclonal antibody to SSH3BP1 (ABI1) (aa483-494) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IHC and WB.
Target
Human ABI1 / SSH3BP1
Synonyms
ABI1 | Abelson interactor 1 | Abl-interactor protein 1 long | ABI-1 | Abl-interactor 1 | Abl interactor 1 | Abl-binding protein 4 | ABLBP4 | NAP1BP | Nap1 binding protein | SSH3BP1 | Nap1-binding protein | E3B1 | Eps8-binding protein | Interactor protein AblBP4 | SSH3BP
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Guinea pig, Horse, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human ABI1 (483-494 aa WYEGVCNRVTGL), different from the related rat and mouse sequences by one amino acid.
Epitope
aa483-494
Specificity
Widely expressed, with highest expression in brain. .
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ABI1 / SSH3BP1
Q8IZP0 NM_005470 NP_005461.2

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Featured Products

ABI1 / SSH3BP1 Antibody - Detection of Human ABI1 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ABI1 used at a dilution of 1:200 (1 ug/ml).
ABI1 / SSH3BP1 Antibody (aa325‑375) LS‑C288019
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
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ABI1 / SSH3BP1 Antibody (clone 1B9) LS‑C179409
Species: Human, Mouse, Hamster
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
ABI1 / SSH3BP1 Antibody - Anti-ABI1 / ABI-1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC‑plus™ ABI1 / SSH3BP1 Antibody (aa325‑375) LS‑B11045
Species: Human
Applications: IHC, IHC - Paraffin
Product
ABI1 / SSH3BP1 Antibody (C‑Terminus) LS‑C47047
Species: Human, Mouse
Applications: Western blot, ELISA
Product
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Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot, Immunoprecipitation
CRBN / Cereblon Protein - 12.5% SDS-PAGE of human CRBN stained with Coomassie Blue
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See all ABI1 / SSH3BP1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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